长江中下游麦区小麦地方品种HMW-GS遗传多样性分析

采用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)方法,对长江中下游麦区1 120份小麦地方品种的高分子量麦谷蛋白亚基(HMW-GS)的组成进行了研究.结果表明:在供试材料中,Glu-1位点共有14种等位基因变异,其中Glu-A1位点3种,Glu-B1位点5种,Glu-D1位点6种.亚基null、7+8和2+12在各自的位点上出现频率最高,分别达到了99.38%、97.86%和98.03%.亚基组成类型共有14种,主要为null/7+8/2+12,频率高达95.90%,其中有8份材料具有1、2*、14+15等优质亚基.

Genetic Diversity of HMW-GS Composition in Wheat Landraces from Middle and Low Yangtze Valleys Winter Wheat Region

In order to understand the genetic diversity of wheat landraces in middle and low Yangtze valleys winter wheat region, 1 120 wheat landraces in middle and low Yangtze valleys winter wheat region (Jianghuai plain sub-area, Yanjianghubin sub-area, The south mountain area of Zhewan subarea and Xianggan sub-area) were analyzed using SDS-PAGE for the allelic variances of high molecular weight glutenin subunits (HMW-GS).The results revealed that out of all material taken under consideration there were 14 alleles on Glu-1 loci,including 3 alleles in Glu-A1 locus, 5 alleles on Glu-Bllocus,and 6 alleles on Glu-Dl locus.The frequencies of null, 7-I-8 and 2+12 alleles were the highest on the Glu-A1,Glu-Bl and Glu-Dl loci, which were 99.38%,97.86% and 98.03% respectively.14 subunit composition types were found,including 8 landraces with 1,2^*,14+15 subunits performed significant implications regarding, and the predominant type was null/7+8/2+12 with the frequency 95.90%.