A versatile targeted metabolomics method for the rapid quantification of multiple classes of phenolics in fruits and beverages |
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Authors: | Vrhovsek Urska Masuero Domenico Gasperotti Mattia Franceschi Pietro Caputi Lorenzo Viola Roberto Mattivi Fulvio |
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Affiliation: | Food Quality and Nutrition Department, Fondazione Edmund Mach , IASMA Research and Innovation Centre, via E. Mach 1, 38010 San Michele all'Adige, Italy. |
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Abstract: | Compelling evidence of the health benefits of phenolic compounds and their impact on food quality have stimulated the development of analytical methods for the identification and quantification of these compounds in different matrices in recent years. A targeted metabolomics method has been developed for the quantification of 135 phenolics, such as benzoates, phenylpropanoids, coumarins, stilbenes, dihydrochalcones, and flavonoids, in fruit and tea extracts and wine using UPLC/QqQ-MS/MS. Chromatography was optimized to achieve separation of the compounds over a period of 15 min, and MRM transitions were selected for accurate quantification. The method was validated by studying the detection and quantification limits, the linearity ranges, and the intraday and interday repeatability of the analysis. The validated method was applied to the analysis of apples, berries, green tea, and red wine, providing a valuable tool for food quality evaluation and breeding studies. |
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