Identification of<Emphasis Type="Italic">Pseudomonas viridiflava</Emphasis> on tomato by traditional methods and enzyme-linked immunosorbent assay |
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Authors: | Email author" target="_blank">Yesim?AysanEmail author Nilufer?Yildiz Fatima?Yucel |
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Institution: | (1) Faculty of Agriculture, Plant Protection Department, Cukurova University, 01330 Balcali, Adana, Turkey;(2) Research Institute for Genetic Engineering and Biotechnology, TUBITAK, 41470 Gebze, Kocaeli, Turkey |
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Abstract: | Pseudomonas viridiflava is one of the causal agents of tomato stem necrosis in the eastern Mediterranean region of Turkey. The bacterium causes general
wilting, yellowing of tomato plants, dark blotches on the pruning sites of the stem, browning, and hollowing of the pith.P. viridiflava strains, isolated from Antakya and Mersin, were identified by traditional methods and indirect enzyme-linked immunosorbent
assay (ELISA). For indirect-ELISA, polyclonal antisera were produced against a regional isolate ofP. viridiflava (AD-OZ 3a). Using indirect-ELISA, the pathogenic bacterium was identified rapidly and safely from both pure culture and inoculated
plants in 2 days. There was no cross reaction with other stem necrosis pathogens. With indirect-ELISA, the lower limit forP. viridiflava detection in pure culture was 103 colony-forming units per milliliter.
http://www.phytoparasitica.org posting Feb. 11, 2004. |
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Keywords: | ELISA Pseudomonas identification stem necrosis tomato |
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