野生天山马鹿哈密种群微卫星遗传多样性 |
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引用本文: | 夏米西丁.阿不都热依木艾斯卡尔.买买提,布左拉·吐尔逊孜拉吉古丽·西克然木,阿依努尔·阿卜杜艾尼马合木提·哈力克.野生天山马鹿哈密种群微卫星遗传多样性[J].野生动物,2014(1):38-44. |
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作者姓名: | 夏米西丁.阿不都热依木艾斯卡尔.买买提 布左拉·吐尔逊孜拉吉古丽·西克然木 阿依努尔·阿卜杜艾尼马合木提·哈力克 |
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作者单位: | [1]新疆大学生命科学与技术学院,乌鲁木齐830046 [2]新疆师范大学生命科学学院,乌鲁木齐830054 |
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基金项目: | 国家自然科学基金项目(31360266;31060152);新疆维吾尔自治区自然科学基金项目(2010211A02);新檀大学自治区重点学科--动物学科资助 |
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摘 要: | 为了解野生天山马鹿哈密种群的微卫星多态性,本文选用5对微卫星标记引物,利用粪便DNA分析法、PCR和复合电泳银染技术,通过计算基因频率(JP)、有效等位基因数(M)、群体杂合度(1te)、多态信息含量(PIC)和遗传距离,对冬季采集的201份天山马鹿粪便进行了研究,评价了其遗传多样性和系统进化分析。结果表明:成功提取I)NA的194份粪便分属140个体;种群平均等位基因4.200±0.837;平均有效等位基因数3.2167±0.0236;平均多态信息含量0.6332±0.0017;平均期望杂合度0.694±0.035;平均观察杂合度0.792±O.063。整个种群没显著偏离Hardy—Weinberg平衡,种群剧定系数Fis为一0.238。从此可见,野生天山马鹿哈密种群具有比较丰富的遗传多样性.
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关 键 词: | 遗传多样性 野生天山马鹿 微卫星 新握哈密 |
Microsatellite Genetic Diversity of Komul Population of Wild Tianshan Red Deer |
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Institution: | Shamshidin Abduriyim1 Askar Mamat2 Buzohra Tursun1 Zilajigvl Ekram1 Aynur Abdughene Mahmut Halik (1. College of Life Sciences and Technology, Xinjiang University, Urumqi, Xinjiang, 830046, China; 2. College of Life Science, Xinjiang Normal University, Urumqi, Xinjiang, 830054, China) |
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Abstract: | Abstract: We assessed the genetic diversity of the wild Tianshan red deer population in Komut, Xinjiang. We collected 201 fecal samples of red deer from 3 Komul herds and analyzed genetic di- versity using the fecal DNA analysis method and PCR and multiplex gel electrophoresis of silver staining technology with 5 pairs of microsatellite markers. These markers were highly polymorphic loci with rich genetic diversity in the Komul population. The total of 201 fecal samples from 3 groups were assigned to 140 individuals. Mean numbers of alleles and effective alleles(Ne) were 4. 20 ± 0. 84 and 3. 217±0. 024, respectively. The average polymorphic information content (PIC) was 0. 633 ± 0. 002, and expected heterozygosity (He) and mean observed heterozygosity (Ho) were 0. 694 ±0. 035 and 0. 792 ±0. 063, respectively. The population had not deviatedsignificantly from Hardy-Weinberg Equilibrium( HWE)and the inbreeding coefficient was -0. 238. Overall, a high level of genetic diversity was observed in the Tianshan red deer population in Komul. |
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Keywords: | Genetic diversity Wild Tianshan red deerMicrosatellite Komul in Xinjiang |
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