| 绵羊精子膜蛋白PH-20 mRNA在睾丸和附睾中表达的分析 |
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| 引用本文： | 娜仁花,旭日干.绵羊精子膜蛋白PH-20 mRNA在睾丸和附睾中表达的分析[J].中国兽医学报,2006,26(2):204-206. |
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| 作者姓名： | 娜仁花 旭日干 |
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| 作者单位： | 1. 内蒙古农业大学,动物科学与医学学院,内蒙古,呼和浩特,010018;哺乳动物生殖生物学与生物技术教育部重点实验室,内蒙古大学,生命科学学院实验动物研究中心,内蒙古,呼和浩特,010021
2. 哺乳动物生殖生物学与生物技术教育部重点实验室,内蒙古大学,生命科学学院实验动物研究中心,内蒙古,呼和浩特,010021 |
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| 摘 要： | 提取绵羊睾丸总RNA，并以此为模板，采用RT—PCR技术扩增出精子表面特异性蛋白PH-20的cDNA。应用T／A克隆策略，将扩增的PH-20基因克隆入T栽体，通过酶切和测序进行鉴定，结果表明，PH-20 mRNA在绵羊睾丸和附睾中均有表达。
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| 关 键 词： | 精子膜蛋白 绵羊 |
| 文章编号： | 1005-4545(2006)02-0204-03 |
| 修稿时间： | 2005年6月10日 |
Sperm Surface Protein PH-20 mRNA Expression in the Testis and Epididymis of Ram |
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| NaRen Hua,XuRi Gan.Sperm Surface Protein PH-20 mRNA Expression in the Testis and Epididymis of Ram[J].Chinese Journal of Veterinary Science,2006,26(2):204-206. |
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| Authors: | NaRen Hua XuRi Gan |
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| Abstract: | The sperm surface specific protein PH-20 is a single-chain protein,anchored in the plasma membrane with a glycosyl phosphatidylinositol(GPI) linkage and localized on the plasma membrane of the posterior sperm head region and inner acrosomal membrane.It has been shown to possess hyaluronidase activity,which is play an important role in the fertilization process.In this study,total RNA was prepared from sheep testis.cDNA fragment was amplified by RT-PCR using specific primers,and then was cloned into pGM-T Easy vector.Inserted PH-20 gene was sequenced.Thus,the PH-20 gene was proved to be expressed in both the testis and epididymis of sheep. |
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| Keywords: | PH-20 RT-PCR |
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