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| 堆型艾美耳球虫子孢子和鸡十二指肠上皮细胞的共同抗原的研究 | |
| 引用本文： | 王承民,魏刚才,秦建华,何宏轩,刘丽艳,齐永华,吴艳云. 堆型艾美耳球虫子孢子和鸡十二指肠上皮细胞的共同抗原的研究[J]. 畜牧兽医学报, 2007, 38(8): 877-880 |
| 作者姓名： | 王承民 魏刚才 秦建华 何宏轩 刘丽艳 齐永华 吴艳云 |
| 作者单位： | 1. 河南科技学院细胞工程重点实验室,新乡,453003 2. 河北农业大学动物科技学院,保定,071001 3. 中国科学院动物研究所,北京,100080 |
| 基金项目： | 国家自然科学基金资助项目(30571394) |
| 摘 要： | 以抗堆型艾美耳球虫子孢子的单抗EASP-3G3作为工具,对鸡各段消化道上皮细胞切片和子孢子进行免疫组化染色,并利用蛋白质印迹技术来检测单抗所识别子孢子可溶性抗原的分子量,来确定子孢子和十二指肠上皮细胞之间是否存在共同抗原。结果表明单抗只与十二指肠上皮细胞发生反应,而与其他肠段无染色反应。而且单抗所识别的可溶性抗原分子量为35~48 ku。抗子孢子的单抗同时与十二指肠上皮细胞和子孢子反应,而不与其他肠段反应,证明堆型艾美耳球虫寄生的位点特异性与十二指肠上皮细胞表面的某种抗原分子有内在的关系。 |
| 关 键 词： | 鸡 堆型艾美耳球虫子孢子 十二指肠上皮细胞 共同表位 |
| 文章编号： | 0366-6964（2007）08-0877-04 |
| 修稿时间： | 2007-01-30 |
Common Antigen Epitopes on Eimeria acervulina Sporozoites and Duodenum Epithelium of Chickens |
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| WANG Cheng-min,WEI Gang-cai,QIN Jian-hua,HE Hong-xuan,LIU Li-yan,QI Yong-hua,WU Yan-yun. Common Antigen Epitopes on Eimeria acervulina Sporozoites and Duodenum Epithelium of Chickens[J]. Chinese Journal of Animal and Veterinary Sciences, 2007, 38(8): 877-880 | |
| Authors: | WANG Cheng-min WEI Gang-cai QIN Jian-hua HE Hong-xuan LIU Li-yan QI Yong-hua WU Yan-yun |
| Affiliation: | 1. Cell Engineering Key Laboratory, Henan Institute of Science and Technology, Xinxiang 453003, China ; 2. College of Animal Science and Technology, Hebei Agricultural University, Baoding 071001, China ; 3. Institute of Zoology, Chinese Academy of Sciences , Beijing 100080, China |
| Abstract: | An monoclonal antibody(EASP-3G3) produced previously against Eimeria acervulina sporozoites was used as tool to detect common antigen both on duodenum epithelium and sporozoites.The weight of soluble antigen recognized by MAb were determined by Western blot analysis.Immuno histochemistry and immunofluorescence techniques were used to determine whether common epitopes exist on sporozoites and duodenum epithelium.The results showed that the anti-sporozoites MAb was found to react specifically with duodenum epithelium,in addition to the other parts of the digestive tract.In Western blot after sodium dodecyl sulfate-polyacrylamide gel electrophoresis,the monoclonal antibody recognized an 35-48 ku protein.The results above suggested that the interrelationship between specific site invaded by Eimeria acervulina sporozoites and certain antigen existed on the surface of duodenum epithelium,can exist during the infection. |
| Keywords: | chicken Eimeria acervulina sporozoites duodenum epithelium common epitopes |
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