越冬白蜡虫微生物多样性分析

目的]了解白蜡虫越冬时体内微生物的多样性,比较昆明与长春越冬虫体内微生物种类和数量的差异,为了解白蜡虫低温适应机制提供有用信息。方法]采用Miseq高通量测序方法对昆明越冬雌成虫(KM)和长春越冬雌成虫(CC)体内细菌16s rRNA及真菌ITS基因进行测序,利用Usearch软件进行细菌和真菌OTU(Operational Taxonomic Unit)划分,并利用Mothur软件对OTU进行分类学分析和多样性指数分析。结果]细菌KM和CC样品中共得到344个OTU,真菌KM和CC样品中共得到230个OTU。细菌共鉴定到15门、137属,在KM中乳球菌属占主要比例(29.78%),而在CC中立克次氏体占主要比例(55.77%),真菌中共鉴定到6门、83属,在KM中仅鉴定到41个属,而CC中鉴定到75个属,其中线虫草科无法归类的属在2个样品中均为优势菌群,但CC中含量远低于KM。结论]昆明与长春越冬虫体内细菌及真菌种类和数量均不相同,与昆明越冬虫不同,需要应对极端低温的长春越冬虫体内立克次氏体为优势菌群。

Microorganism Diversity Analysis of Overwintering Chinese White Wax Scale Insect

Objective] The study aimed to investigate the microorganism diversity and compare the difference of microorganism between overwintering individuals in Kunming and Changchun. Method] The bacteria 16S rRNA and the fungi internal transcribed space (ITS) of overwintering individuals in Kunming (KM) and Changchun (CC) were sequencing by MiSeq high-throughput sequenced method. The bacterial and fungal Operational Taxonomic Units (OTUs) were obtained by the Usearch software, and the Mothur software was used to calculate and analyze the taxonomy and alpha diversity. Result] According to 344 OTUs obtained from KM and CC bacteria samples, 15 phyla and 137 genera were identified. Meanwhile, 6 phyla and 83 genera were identified from 230 OTUs obtained from KM and CC fungi samples. In KM the dominant bacteria, Lactococcus accounted for 29.78%. And in CC Rickettsia was dominant (55.77%). Unclassified Ophiocordycipitaceae was dominant both in KM and CC, but its proportion in CC was much lower than in KM. Conclusion] In this study, lots of differences were found between Kunming and Changchun overwintering individuals on the bacteria and fungi category and quantity. In comparison of KM bacteria sample, Rickettsia became the dominant genus in CC.