Effect of phytohormones on in vitro shoot multiplication and rooting of lime Citrus aurantifolia (Christm.) Swing |
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| Institution: | 1. State Key Lab for Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, China;2. College of Horticulture Science, Nanjing Agricultural University, China;3. Department of Food Science, Aarhus University, Denmark;4. Department of Plant and Environmental Sciences, University of Copenhagen, Denmark;1. Department of Discovery Chemistry, Bristol-Myers Squibb, Research and Development, PO Box 5400, Princeton, NJ 08543-5400, United States;2. Department of Cardiovascular Diseases, Bristol-Myers Squibb, Research and Development, PO Box 5400, Princeton, NJ 08543-5400, United States;3. Department of Pharmaceutical Candidate Optimization, Bristol-Myers Squibb, Research and Development, PO Box 5400, Princeton, NJ 08543-5400, United States;1. College of Materials Science and Engineering, Beijing University of Technology, Beijing, 100124, PR China;2. School of Materials Science and Engineering, Taiyuan University of Technology, Taiyuan, 030024, PR China;1. Department of Horticulture, Faculty of Agricultural Sciences and Technology, Bahauddin Zakariya University, Multan, Pakistan;2. Citrus Research and Education Center, Horticultural Sciences Department, Institute of Food and Agricultural Sciences, University of Florida, Lake Alfred, FL, United States;3. Department of Agronomy, Faculty of Agricultural Sciences and Technology, Bahauddin Zakariya University, Multan, Pakistan;4. Department of Soil science, Faculty of Agricultural Sciences and Technology, Bahauddin Zakariya University, Multan, Pakistan;5. Equpie “Structure Evolutive des Agrumes, Polyploidie et Amelioration Genetique”, SEAPAG-UM AGAP-Department BIOS-CIRAD Station de Roujol, 97170, Petit Bourg, Guadeloupe, France;1. Department of Chemistry, Faculty of Science, Chiang Mai University, 239 Huay Kaew Rd, Suthep, Muang Chiang Mai 50200 Thailand;2. Center of Advanced Materials for Printed Electronics and Sensors, Materials Science Research Center, Faculty of Science, Chiang Mai University, Suthep Muang Chiang Mai 50200 Thailand;3. National Metal and Materials Technology Center, 114 Thailand Science Park, Paholyothin Rd., Klong 1, KlongLuang Pathumthani Thailand;1. Department of Horticultural Biotechnology, College of Life Sciences, Kyung Hee University, Yongin 17104, Republic of Korea;2. Department of Plant and Environmental New Resources, College of Life Sciences, Kyung Hee University, Yongin 17104, Republic of Korea |
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| Abstract: | Multiple shoots were produced from node explants of lime (Citrus aurantifolia (Christm.) Swing) on MS medium supplemented with 6-benzylaminopurine (BAP), 6-furfurylaminopurine (kinetin) and α-naphthaleneacetic acid (NAA). The highest number of shoots, nine shoots per node, were produced on a medium containing 2 mg l?1 BAP (8.8 μM), 1 mg l?1 kinetin (4.6 μM) and 1 mg l?1 NAA (5.4 μM). Depending on the concentration of BAP and kinetin, NAA either inhibited, stimulated or did not affect shoot multiplication, which also depended on the cytokinin level. Maximum shoot length was obtained from treatments containing 0.5 mg l?1 BAP (2.2 μM) combined with 1 mg l?1 kinetin (4.6 μM) and 0.5 mg l?1 NAA (2.7 μM). The largest leaves of resultant shoots were produced on a medium containing 0.5 mg l?1 each of kinetin (2.3 μM) and NAA (2.7 μM). Transferring in vitro shoots to rooting media containing indole-3-butyric acid (IBA) and NAA produced complete plantlets. The highest rooting percentage was obtained on a medium containing either 1 mg l?1 NAA (5.4 μM) alone or 0.5 mg l?1 NAA (2.7 μM) combined with 2 mg l?1 IBA (9.6 μM), whereas the highest number of roots were produced on a treatment containing both 2 mg l?1 NAA (10.8 μM) and 2 mg l?1 IBA (9.6 μM). Roots elongated most on treatments containing 0.5 mg l?1 of either NAA (2.7 μM) or IBA (2.4 μM). Shoot growth associated with the rooting phase was the highest in response to 2 mg l?1 IBA (9.6 μM) or 0.5 mg l?1 NAA (2.7 μM). Plantlets that survived acclimatization, 82%, exhibited normal growth in soil under greenhouse conditions. |
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