Micropropagation of the strawberry tree,Arbutus unedo L. |
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| Affiliation: | 1. Laboratorio de Biología, Instituto Tecnológico de Santo Domingo, República Dominicana;2. Agricultural and Plant Biochemistry and Proteomics Research Group, Dept. of Biochemistry and Molecular Biology, University of Córdoba, Agrifood Campus of International Excellence (ceiA3), 14071 Córdoba, Spain;3. Departamento de Fitoquímica, Dirección de Investigación de la Facultad de Ciencias Químicas de la Universidad Nacional de Asunción, Paraguay;4. Department of Forestry Engineering, ETSIAM, University of Córdoba, Agrifood Campus of International Excellence (ceiA3), 14071 Córdoba, Spain;1. Shandong Forestry Research Academy, Jinan, 250014, China;2. Agricultural Faculty, Yamaguchi University, Japan;3. Forestry College, Shandong Agricultural University, Taian, China |
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| Abstract: | Actively growing shoots of potted greenhouse-grown strawberry tree (Arbutus unedo L.) were initially sterilised and established in basal woody plant medium containing 11.1 μM BA. Optimum shoot proliferation was achieved on a basal WPM containing MS vitamins, sucrose, agar and 22.2 μM BA. Microshoots rooted successfully in basal in vitro medium containing 10 μM IBA or IAA, but their survival rate during acclimatisation was low. Addition of a mixture 1 part peat:4 parts perlite in the basal in vitro rooting medium (1:1 v/v) containing 10 μM IAA resulted in high rooting percentage and plantlets with branched roots. These plantlets were successfully acclimatised. This novel rooting medium can be exploited further due to its potential in commercial applications. |
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