Factors affecting the shoot multiplication of Persian walnut (Juglans regia L.) |
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| Affiliation: | 1. Fars Research Centre for Natural Resources and Animal Science, P.O. Box 617, Shiraz, 71555, Islamic Republic of Iran;2. Department of Crop Science, Horticulture and Forestry, University College Dublin, Belfield, Dublin 4, Ireland;1. Dept. of Pharmaceutics, School of Pharmaceutical Education and Research, Jamia Hamdard, New Delhi, India;2. Dept. of Wood Processing, Mendel University, Brno, Czech Republic;3. Department of Production Management and Engineering, Warsaw University of Life sciences, Poland;4. Dabur Research Foundation, Ghaziabad, India;5. DPSRU, New Delhi, India;1. Department of Biology, Faculty of Science, Golestan University, Gorgan, Iran;2. Molecular Physiology Department, Agricultural Biotechnology Research Institute of Iran, (ABRII), AREEO, 3135933151 Karaj, Iran;3. Department of Statistics, Faculty of Science, Gorgan, Iran;1. State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Guangzhou 510006, China;2. Department of Gastric Surgery, Sun Yat-Sen University Cancer Center, Guangzhou 510006, China;3. Department of Hematology Oncology, Sun Yat-Sen University Cancer Center, Guangzhou 510006, China;4. Department of the VIP Region, Sun Yat-Sen University Cancer Center, Guangzhou 510006, China;1. Laboratoire de Pharmacognosie, UMR/CNRS 8638, Université Paris Descartes, Sorbonne Paris Cité, Faculté des Sciences Pharmaceutiques et Biologiques, 4 Avenue de l’Observatoire, 75006 Paris, France;2. Centre de Recherche de Gif, Institut de Chimie des Substances Naturelles (ICSN), CNRS, 1, Avenue de la Terrasse, 91198 Gif sur Yvette Cedex, France;3. Laboratoire des Plantes Médicinales de Nouméa – CNRS, Centre IRD de Nouméa, BP683 98845 Nouméa Cedex, Nouvelle Calédonie, France;1. School of Land and Food, University of Tasmania, Australia;2. Department of Botanical and Environmental Sciences, Guru Nanak Dev University, Amritsar 143005, Punjab, India;3. Department of Agronomy, Gomal University, Pakistan |
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| Abstract: | A number of experiments were conducted to identify suitable procedure for in vitro shoot multiplication of Persian walnut (Juglans regia L.). Three different nutrient media (DKW, MS and WPM) and three different gelling agents (Phytagel, Difco Bacto agar and a mixture of Phytagel and Difco Bacto agar) were studied in the first experiment. Driver and Kuniyuki walnut (DKW) medium solidified with 2.2 g l−1 Phytagel was found optimum. Performance of explants was better on DKW medium than on MS and WPM. The DKW and MS media were not significantly different from each other, but both of them were significantly better than WPM, which was a very poor medium for this species. Phytagel alone was significantly better than Difco Bacto agar or Phytagel combined with Difco Bacto agar. In another experiment different concentrations of BA were studied. Medium containing 1.0 mg l−1 BA and 0.01 mg l−1 IBA was the best, although medium containing 0.6 and 0.8 mg l−1 BA were also successful, and utilisation of 0.4 mg l−1 BA and 0.01 mg l−1 IBA was optimum for shoot elongation. Application of different kinds of auxins (IAA, IBA and NAA at 0.01 or 0.1 mg l−1) with 1.0 mg l−1 BA were also studied. Media containing IBA were significantly better than media containing IAA for shoot fresh weight, but neither of them was significantly different from media containing NAA. Application of 0.01 mg l−1 or 0.1 mg l−1 auxin, with 1.0 mg l−1 BA, was not significantly different for shoot multiplication of Persian walnut. The morphology of shoots on media containing 0.01 mg l−1 IBA was the best. |
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