Isolation and Characterization of Efficient Active Compounds Using High-Performance Centrifugal Partition Chromatography (CPC) from Anti-Inflammatory Activity Fraction of Ecklonia maxima in South Africa |
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Authors: | Hyun-Soo Kim Jun-Geon Je Hyesuck An Kyunghwa Baek Jeong Min Lee Mi-Jin Yim Seok-Chun Ko Ji-Yul Kim Gun-Woo Oh Min-Cheol Kang Young Min Ham You-Jin Jeon Dae-Sung Lee |
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Affiliation: | 1.National Marine Biodiversity Institute of Korea, 75, Jangsan-ro 101 gil, Janghang-eup, Seocheon 33662, Korea; (H.-S.K.); (H.A.); (K.B.); (J.M.L.); (M.-J.Y.); (S.-C.K.); (J.-Y.K.); (G.-W.O.);2.Department of Marine Life Science, School of Marine Biomedical Sciences, Jeju National University, Jeju 63243, Korea;3.Research Group of Food Processing Research Division of Strategic Food Technology, Wanju-gun 55365, Korea;4.Korea Jeju Biodiversity Research Institute, Jeju Technopark, Jeju 63608, Korea; |
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Abstract: | Ecklonia maxima is a brown seaweed, which is abundantly distributed in South Africa. This study investigated an efficient approach using high-performance centrifugal partition chromatography (HPCPC), which has been successfully developed for the isolation and purification of phlorotannins, eckmaxol, and dieckol from the ethyl acetate fraction of E. maxima (EEM). We evaluated EEM for its inhibitory effect against lipopolysaccharide (LPS)-induced inflammatory responses in zebrafish embryos. The separation of eckmaxol and dieckol from samples of EEM using HPCPC was found to be of high purity and yield under an optimal solvent system composed of n-hexane:ethyl acetate:methanol:water (2:7:3:7, v/v/v/v). To evaluate the anti-inflammatory efficacy of EEM containing active compounds, zebrafish embryos exposed to LPS were compared with and without EEM treatment for nitric oxide (NO) production, reactive oxygen species (ROS) generation, and cell death two days after fertilization. These evaluations indicate that EEM alleviated inflammation by inhibiting cell death, ROS, and NO generation induced by LPS treatment. According to these results, eckmaxol and dieckol isolated from brown seaweed E. maxima could be considered effective anti-inflammatory agents as pharmaceutical and functional food ingredients. |
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Keywords: | Ecklonia maxima high-performance centrifugal partition chromatography eckmaxol dieckol anti-inflammatory zebrafish |
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