Assessment of homozygosity and fertility in meiotic gynogens of the European sea bass (Dicentrarchus labrax L.) |
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Affiliation: | 1. University of South Bohemia in Ceske Budejovice, Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, Zátiší 728/II, 389 25 Vodňany, Czech Republic;2. Department of Aquaculture, Szent István University, Páter Károly u. 1, 2100 Gödöllő, Hungary;3. Department of Lake and River Fisheries, University of Warmia and Mazury, Oczapowskiego 2, 10-719 Olsztyn, Poland;1. Department of Physiological Sciences, Laboratory of Zoophysiology and Comparative Biochemistry, Federal University of São Carlos (UFSCar), P.O. Box 676, Via Washington Luis, Km 235, 13565-905 São Carlos, SP, Brazil;2. Joint Graduate Program in Physiological Sciences, Federal University of São Carlos, SP (UFSCar), São Paulo State University (UNESP), Araraquara, SP, Brazil;3. Department of Zoology and Botany, IBILCE, São Paulo State University (UNESP), São José do Rio Preto, SP, Brazil;4. National Institute of Science and Technology in Comparative Physiology (FAPESP/CNPq), Sao Paulo, Brazil |
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Abstract: | Analysis of 5–6 microsatellite loci was used to measure the increment of homozygosity in two meiogynogenetic progenies (A and B) of sea bass with respect to their mother. In progeny A and B, 20% and 12% of the meiogynogens retained heterozygosity for all investigated maternal loci, respectively, while complete homozygosity was observed only in 6% of B and in none of A, indicating the occurrence of significant allelic recombination during meiosis. The overall increment of homozygosity for the investigated loci was 27% for A and 47% for B. Although survival at hatching of meiogynogens was about half that of controls, they subsequently grew as controls and displayed the same onset of puberty and reproductive potential at adulthood during three consecutive years when crossed between themselves (11 crosses) or with control fish (13 crosses). In particular, meiogynogenetic females (n=24) underwent vitellogenesis and yielded eggs of good quality upon stimulation with LH–RH analogue similarly to normal fish. Sperm released by meiogynogenetic males (n=23) was equivalent to that of controls in terms of volume, quality and fertilization capability.Second generation meiogynogens were obtained by chromosome set manipulation from meiogynogenetic females and found to be morphologically normal at 3 years of age. Interestingly, under our culture conditions, the percentage of meiogynogenetic males in the second generation was 7% as opposed to 39% in the first generation. |
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