Gynogenesis induction and sex determination in the Eurasian perch,Perca fluviatilis |
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| Affiliation: | 1. University of South Bohemia in Ceske Budejovice, Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, Zátiší 728/II, 389 25 Vodňany, Czech Republic;2. Department of Aquaculture, Szent István University, Páter Károly u. 1, 2100 Gödöllő, Hungary;3. Department of Lake and River Fisheries, University of Warmia and Mazury, Oczapowskiego 2, 10-719 Olsztyn, Poland;1. École Normale Supérieure, PSL Research University, Département de chimie, 24 rue Lhomond, 75005 Paris, France;2. Sorbonne Universités, UPMC Univ Paris 06, LBM, 4 Place Jussieu, 75005 Paris, France;3. CNRS, LBM, UMR 7203, 75005 Paris, France;4. Department of Chemistry and Pharmacy, University of Erlangen-Nürnberg, Egerlandstr. 1, 91058 Erlangen, Germany |
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| Abstract: | In the present study, we used meiotic gynogenesis, widely used in studies on sex determination, to confirm female homogamety in Eurasian perch, Perca fluviatilis. Sperm irradiated with UV for 400 s was used to artificially fertilized eggs. The diploid of the resulting embryos was restored by a heat shock (30 °C) applied to the eggs 5 min postfertilization, for 25 min. Fertilization (ranging between 45% and 75%) and survival rates at hatching (ranging between 3.4% and 46.6%) were not significantly different (P>0.05) between the diploid control and gynogenetics. The diploid controls and two batches of gynogenetics contained 100% diploid larvae, whereas two other batches of gynogenetics contained 6.7% and 10.0% triploid larvae. The sex ratios of the diploid controls were not significantly different from 1:1, whereas all gynogenetic families were 100% female. These results confirm female homogamety in Eurasian perch, demonstrated by the use of hormonally mascilinized breeders in a previous study. |
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