| 川产绿茶中总黄酮含量的测定研究 |
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| 引用本文： | 卓莉,阮尚全,罗海燕. 川产绿茶中总黄酮含量的测定研究[J]. 安徽农业科学, 2010, 38(3): 1228-1228,1244. DOI: 10.3969/j.issn.0517-6611.2010.03.050 |
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| 作者姓名： | 卓莉 阮尚全 罗海燕 |
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| 作者单位： | 内江师范学院化学与生命科学学院,四川内江,641112;内江师范学院化学与生命科学学院,四川内江,641112;内江师范学院化学与生命科学学院,四川内江,641112 |
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| 摘 要： | ［目的］研究川产绿茶中黄酮含量的测定方法。［方法］采用超声提取和分光光度法，研究黄酮的提取方法和测定方法。［结果］芦丁浓度在8-48 μg/ml范围内，吸光度值与浓度的回归方程为A=0.012 23C-0.004 05，相关系数r=0.999 9，加标平均回收率为100.07%，RSD为2.64%。在50 ℃下，用浓度76%乙醇在超声功率为699.3 W的条件下提取黄酮类化合物的效果最佳。［结论］与贵州产绿茶相比，川产绿茶黄酮类化合物含量较高。
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| 关 键 词： | 分光光度法 绿茶 总黄酮 |
Study on Determination of Flavonoids in Sichuan Green Tea by Spectrophotometry |
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| ZHUO Li et al. Study on Determination of Flavonoids in Sichuan Green Tea by Spectrophotometry[J]. Journal of Anhui Agricultural Sciences, 2010, 38(3): 1228-1228,1244. DOI: 10.3969/j.issn.0517-6611.2010.03.050 |
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| Authors: | ZHUO Li et al |
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| Affiliation: | College of Chemistry and Life Science;Neijiang Normal University;Neijiang;Sichuan 641112 |
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| Abstract: | [Objective] The determination of total flavonoids in the green tea was researched.[Method]The flavonoids was extracted by ultrasonic power and was determined by spectrophotometry.[Result] The linear relationship of rutin was in the range of 8-48 μg/ml. The regression equation was A=0.012 23C-0.004 05, and the correlation coefficient r reached 0.999 9.The average recovery was 100.07%(RSD=2.64%). The optimum condition was as follows: 50 ℃, 699.3 W ultrasonic power, and 76% alcohol. [Conclusion]The content of flavoniods in Sichuan green tea was higher than that of Guizhou green tea. |
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| Keywords: | Spectrophotometry Green tea Flavonoids |
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