RAPD分子标记技术在甜高梁基因组研究中的应用

目的]应用RAPD技术对甜高粱丝黑穗病基因进行分子标记研究。方法]以抗病亲本7050B与感病亲本TX622B杂交后的F2代以及抗病甜高粱品种8113和感病甜高粱品种8101为试材，用CrAB法提取DNA，用RAPD分子标记技术对其DNA进行多态性扩增与初步分析，同时对琼脂糖凝胶电泳检测体系进行优化。结果]CTAB法适宜提取DNA。在对抗病亲本7050B与感病亲本TX622B杂交后的F1代进行RAPD标记时，用60个引物进行筛选，其中27个引物扩增出了多态性谱带；应用20个具有多态性扩增谱带的引物对抗病甜高粱品种8113和感病甜高粱品种8101进行RAID分析时，共有7个引物扩增出了差异谱带，分别为S56、S66、S67、S70、S72、S75、S78。结论]该研究为甜高粱优良品种的培育提供科学基础。

Application of RAPD Molecular Marker Technology in Studying Sweet Sorghum Genome

Objective] The study aimed to research the molecular marker of head smut gene of sweet sorghum by RAPD technology.Method] With the F2 from cross between the resistant parent 7050B and the susceptible parent TX622B and the sweet sorghum resistant variety 8113 and susceptible variety 8101 as the tested materials,their DNAs were extracted by CTAB method.RAPD molecular marker technology was used to make the polymorphism amplification and preliminary analysis on their DNA and the detection system of agarose gel electrophoresis were also optimized.Result] CTAB method was suitable to extract DNA.When F2 from cross between the resistant parent 7050B and the susceptible parent TX622B was made for RAPD molecular marker,60 random primers were used to screen and 27 primers amplified out the polymorphism bands.When 20 primers with polymorphism amplification bands were used to make the RAPD analysis on the sweet sorghum resistant variety 8113 and susceptible variety 8101,there were 7 primers that amplified out the difference bands,being S56,S66,S67,S70,S72,S75 and S78 resp.Conclusion] This study provided the scientific foundation for breeding of good varieties of sweet sorghum.