| 牧草细胞壁降解菌的群落结构多样性研究 |
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| 引用本文： | 侯玉洁,徐俊,王淑清,刘昌林,郭小泽,杨宗英,曾柳根,雷小青,熊春贤,邓敏超,许亮清.牧草细胞壁降解菌的群落结构多样性研究[J].中国饲料,2019(19):28-33. |
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| 作者姓名： | 侯玉洁 徐俊 王淑清 刘昌林 郭小泽 杨宗英 曾柳根 雷小青 熊春贤 邓敏超 许亮清 |
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| 作者单位： | 南昌市农业科学院;江西省农业科学院农产品质量安全与标准研究所;南昌市动物疫病预防控制中心;江西省农业科学院畜牧兽医研究所;新建区农业农村局 |
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| 基金项目： | 国家自然基金“SARA影响锦江黄牛胃肠道微生物和宿主代谢的关联研究”(31560647);江西省草食动物创新联盟项目“牛(羊)肉质量保证体系及追溯系统开发”(JXXTCX201702) |
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| 摘 要： | 本试验通过高通量测序法研究了吸附在牧草细胞壁降解菌的群落结构,分别将粉碎后的苜蓿、燕麦草、羊草和稻草茎秆3.0g放入尼龙袋中,降解24h全部取出,洗净后提取总DNA,根据高通量测序要求制样。结果表明:(1)吸附于不同牧草细胞壁中的细菌群落结构丰富度:燕麦草>苜蓿>稻草>羊草;(2)四组牧草样品共鉴定得到16个门,27个纲,37个目,63个科和91个属。门水平上,硬壁菌门、拟杆菌门、螺旋体门、纤维杆菌门和变形菌门为优势菌门。综上,不同牧草吸附的细菌种类和数量存在显著差异。
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| 关 键 词： | 牧草 细胞壁 微生物多样性 高通量测序 |
Study on the degradation bacterial community adhered in forage cell walls |
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| HOU Yujie,XU Jun,WANG Shuqing,LIU Changlin,GUO Xiaoze,YANG Zongying,ZENG Liugen,LEI Xiaoqing,XIONG Chunxian,DENG Minchao,XU Liangqing.Study on the degradation bacterial community adhered in forage cell walls[J].China Feed,2019(19):28-33. |
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| Authors: | HOU Yujie XU Jun WANG Shuqing LIU Changlin GUO Xiaoze YANG Zongying ZENG Liugen LEI Xiaoqing XIONG Chunxian DENG Minchao XU Liangqing |
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| Institution: | (Institute of Nanchang Agricultural Sciences Academy of Agricultural Sciences,Nanchang,Jiangxi Province 330008,China;Institute of Quality Safety and Standards of Agricultural Products,Jiangxi Academy of Agricultural Sciences,Nanchang,Jiangxi Province 330200,China;Institute of Nanchang Animal Disease Control and Prevention Center,Nanchang ,Jiangxi Province 330008,China;Institute of Animal Husbandry and Veterinary,Jiangxi Academy of Agricultural Sciences,Nanchang,Jiangxi Province 330200,China;Agricultural and Rural Bureau of Xinjian County,Nanchang,Jiangxi Province 330100,China) |
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| Abstract: | HOU Yujie;XU Jun;WANG Shuqing;LIU Changlin;GUO Xiaoze;YANG Zongying;ZENG Liugen;LEI Xiaoqing;XIONG Chunxian;DENG Minchao;XU Liangqing(Institute of Nanchang Agricultural Sciences Academy of Agricultural Sciences,Nanchang,Jiangxi Province 330008,China;Institute of Quality Safety and Standards of Agricultural Products,Jiangxi Academy of Agricultural Sciences,Nanchang,Jiangxi Province 330200,China;Institute of Nanchang Animal Disease Control and Prevention Center,Nanchang ,Jiangxi Province 330008,China;Institute of Animal Husbandry and Veterinary,Jiangxi Academy of Agricultural Sciences,Nanchang,Jiangxi Province 330200,China;Agricultural and Rural Bureau of Xinjian County,Nanchang,Jiangxi Province 330100,China) |
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| Keywords: | forage sources cell walls bacterial community high throughput sequencing |
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