| ELISA与HPLC-MS检测玉米中黄曲霉毒素B_1的比较分析 |
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| 引用本文： | 陈琛,王炳彦,钟兴文,杨旭艳,马丹,戚敏,孙照程,陈莹,孔凡虎,华雪妃,章雨竹,陶琳丽,张曦.ELISA与HPLC-MS检测玉米中黄曲霉毒素B_1的比较分析[J].中国饲料,2019(9):87-92. |
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| 作者姓名： | 陈琛 王炳彦 钟兴文 杨旭艳 马丹 戚敏 孙照程 陈莹 孔凡虎 华雪妃 章雨竹 陶琳丽 张曦 |
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| 作者单位： | 云南农业大学动物科学技术学院云南省动物营养与饲料重点实验室;云南省楚雄州牟定县新桥镇畜牧兽医站;大理白族自治州动物卫生监督所;云南省兽药饲料监测所 |
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| 基金项目： | 云南省重大科技专项项目"高原优质肉鸡产业工程化技术研发集成与示范推广"(2016ZA008号);云南省建立农科教相结合新型农业社会化服务体系试点项目"高原优质地方鸡"(2014NG006号);云南省现代农业产业技术体系建设项目"云南省禽蛋产业技术体系建设"(2017KJTX0017) |
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| 摘 要： | 本实验选用30份玉米样品,通过酶联免疫吸附(ELISA)法和高效液相色谱-质谱联用(HPLC-MS)法对玉米中黄曲霉毒素B1含量(AFB1)进行测定比较,得出两种方法的差异性和相关性。其中,使用HPLC-MS外标法定量时,设计两组标准曲线,分别是玉米基质液加标组曲线和纯标组曲线,得到的结果再与ELISA比较。结果显示:用ELISA筛选出的阳性样品,用HPLC-MS法确证亦为阳性,判定结果一致,但具体检出量存在差异。当样品中AFB1含量为2～6μg/kg时,测定结果 HPLC-MS(纯标组)
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| 关 键 词： | 玉米 黄曲霉毒素B1 检测 酶联免疫法 高效液相色谱-质谱法 |
A comparative analyze on the determination of aflatoxin B_1 in maize with ELISA and HPLC-MS method |
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| CHEN Chen,WANG Bingyan,ZHONG Xingwen,YANG Xuyan,MA Dan,QI Min,SUN Zhaocheng,CHEN Ying,KONG Fanhu,HUA Xuefei,ZHANG Yuzhu,TAO Linli,ZHANG Xi.A comparative analyze on the determination of aflatoxin B_1 in maize with ELISA and HPLC-MS method[J].China Feed,2019(9):87-92. |
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| Authors: | CHEN Chen WANG Bingyan ZHONG Xingwen YANG Xuyan MA Dan QI Min SUN Zhaocheng CHEN Ying KONG Fanhu HUA Xuefei ZHANG Yuzhu TAO Linli ZHANG Xi |
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| Institution: | (Faculty of Animal Science and Technology,Yunnan Key Laboratory of Animal Nutrition and Feed Science,Yunnan Agricultural University,Kunming,Yunnan Province 650201,China;Animal Husbandry and Veterinary Station of Xinqiao Town in Mouding County Chuxiong Prefecture of Yunnan Province,Mouding,Yunnan Province 675501,China;Animal Health Supervision Institute of Dali Bai Autonomous Prefecture,Dali,Yunnan Province 671000,China;Veterinary Drug and Feed Monitoring Station of Yunnan Province,Kunming,Yunnan Province 650201,China) |
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| Abstract: | CHEN Chen;WANG Bingyan;ZHONG Xingwen;YANG Xuyan;MA Dan;QI Min;SUN Zhaocheng;CHEN Ying;KONG Fanhu;HUA Xuefei;ZHANG Yuzhu;TAO Linli;ZHANG Xi(Faculty of Animal Science and Technology,Yunnan Key Laboratory of Animal Nutrition and Feed Science,Yunnan Agricultural University,Kunming,Yunnan Province 650201,China;Animal Husbandry and Veterinary Station of Xinqiao Town in Mouding County Chuxiong Prefecture of Yunnan Province,Mouding,Yunnan Province 675501,China;Animal Health Supervision Institute of Dali Bai Autonomous Prefecture,Dali,Yunnan Province 671000,China;Veterinary Drug and Feed Monitoring Station of Yunnan Province,Kunming,Yunnan Province 650201,China) |
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| Keywords: | maize AFB1 detect ELISA HPLC-MS |
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