In situ detection of cellular and abnormal isoforms of prion protein in brains of cattle with bovine spongiform encephalopathy and sheep with scrapie by use of a histoblot technique. |
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Authors: | Kumiko M Kimura Takashi Yokoyama Makoto Haritani Minoru Narita Peter Belleby Julie Smith Yvonne I Spencer |
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Institution: | National Institute of Animal Health, Tsukuba, Ibaraki, Japan. |
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Abstract: | To detect prion protein, brains from 5 cattle naturally affected with bovine spongiform encephalopathy (BSE) and 3 sheep naturally affected with scrapie were examined and compared with brains of normal cattle and sheep using a histoblot technique. The technique enabled the in situ distinctive detection of the cellular (PrP(C)) and abnormal (PrP(Sc)) isoforms of the prion protein. In BSE- or scrapie-affected brains, the Prp(C) signal decreased, especially in those areas where the PrP(Sc) signal was detected. |
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