| 抗氧化剂对银杏愈伤组织褐变的影响 |
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| 引用本文： | 张智,邵菊芳,滕婷婷,张明.抗氧化剂对银杏愈伤组织褐变的影响[J].安徽农业科学,2008,36(30). |
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| 作者姓名： | 张智 邵菊芳 滕婷婷 张明 |
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| 作者单位： | 中国矿业大学化工学院生物工程系,江苏徐州,221116 |
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| 基金项目： | 中国矿业大学科技基金
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中国矿业大学大学生科研训练计划
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| 摘 要： | 目的]研究抗氧化剂对银杏愈伤组织褐变的影响。方法]以银杏的子叶为外植体,以MS+2,4-D 1.0 mg/L+KT 1.5mg/L为基本培养基,对外植体进行愈伤组织的诱导培养,研究银杏愈伤组织继代过程中,不同浓度Vc、柠檬酸、PA 3种抗氧化剂对愈伤组织培养褐变的影响。抗氧化剂对褐变的抑制效果以愈伤组织中叶绿素的含量来表示。结果]3种抗氧化剂对抑制褐变的效果为:Vc>柠檬酸>PA;筛选出最佳抗氧化剂及其浓度为Vc 15 mg/L。结论]抗氧化剂的类型及浓度在抑制银杏愈伤组织褐化过程中起关键作用。
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| 关 键 词： | 银杏 愈伤组织 抗氧化剂 褐化 叶绿素 |
Effects of Antioxidants on Browning of the Ginkgo Callus |
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| Abstract: | Objective] The research aimed to study the effects of antioxidants on browning of the ginkgo callus.Mehtod] Taking the cotyledon of ginkgo as explants,and using MS + 2,4-D 1.0 mg/L+ KT 1.5 mg/L as the basic medium,the callus from the explants were clutivated and the effects of 3 antioxidants Vc,citric acid and PA with different concentrations on browning of the ginkgo callus were studied.Result] The results showed that effects of different antioxidants on browning were different,and the sequence from high to low was Vc>citric acid>PA.The best antioxidant was Vc and its optimal concentration was 15mg/L.Conclusion] The style and content of antioxidants play an important role in inhibiting browning of callus of Ginkgo biloba. |
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| Keywords: | Ginkgo Callus Antioxidant Browning Chlorophyl |
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