| 葡萄细胞培养生产白藜芦醇的研究 |
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| 引用本文： | 邓建平,姜丰,杨国顺.葡萄细胞培养生产白藜芦醇的研究[J].湖南农业大学学报(自然科学版),2009,35(3). |
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| 作者姓名： | 邓建平 姜丰 杨国顺 |
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| 作者单位： | 1. 湖南生物机电职业技术学院,植物科技系,湖南,长沙410127
2. 湖南农业大学,园艺园林学院,湖南,长沙,410128 |
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| 摘 要： | 以巨峰和红地球葡萄的不同组织为试材,利用不同培养基对其愈伤组织进行诱导,测定愈伤组织中的白藜芦醇含量.结果表明:在B5基本培养基附加6 mg/L NAA和0.6 mg/L 6-BA的培养条件下,巨峰葡萄果皮愈伤组织的诱导率最高,达87%.愈伤组织中白藜芦醇的含量均高于其对应外植体中的含量,其中巨峰葡萄果皮愈伤组织中的白藜芦醇含量最高,达11.184 μg/g.经6次继代培养可诱导出生长势强、白藜芦醇含量为14.296 μg/g的巨峰葡萄优良愈伤组织.
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| 关 键 词： | 葡萄 细胞培养 白藜芦醇 |
Using cell culture of grape to produce resveratrol |
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| DENG Jian-ping,JIANG Feng,YANG Guo-shun.Using cell culture of grape to produce resveratrol[J].Journal of Hunan Agricultural University,2009,35(3). |
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| Authors: | DENG Jian-ping JIANG Feng YANG Guo-shun |
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| Institution: | 1.College of Plant Science and Technology;Hunan Biological and Electromechanical Polytechnic;Changsha 410127;China;2.College of Horticulture and Landscape;HNAU;Changsha 410128;China |
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| Abstract: | Taking Kyoho grape and Red Globe grape for materials,using callus at different elementary culture medium,content of res in the different callus were determined. The results indicated that on the medium for the callus growth which is B5 medium added 6 mg/L NAA and 0.6 mg/L 6-BA, the inductivity in Kyoho grape peel callus is the highest(87%),all the res contents in grape callus are higher than that of control. The res content in Kyoho grape peel callus is the highest(11.184 μg/g). After 6 times of subculture,the res,with its content being 14.296 μg/g was used as the Kyoho grape explants of cell culture. |
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| Keywords: | grape cell culture resveratrol |
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