甘薯叶中清除自由基活性物质的提取·保存与定性分析

探讨影响甘薯叶提取物清除不同种类自由基的提取工艺和保存温度，为其应用于抗衰老化妆品提供理论依据。

Extraction Storage and Qualitative Analysis of Free Radicals Scavenging Substances from Sweet Potato Leaves

[Objective] The research aimed to provide reference for the application d extracts from sweet potato leaves in anti-aging cosmetics. [Method] The extraction and storage conditions for free radicals scavenging substances ham sweet potato leaves were optimized by orthogonal test and the bioactive components in extracts were investigated by correlation analysis. [ Result] Sweet potato leaves contain the bioactive substances scavenging DPPH free radi-cal and hydroxyl free radical. Extracting solvent species is the most important factor that influencing extraction yield. The optimal extraction and storage conditions are as following: water as solvent, pH 8.0 of extracting liquid, storage at 25 0C. There is a good positive linear relationship between the content of total phenols in sweet potato leaves and corresponding scavenging rates against both the DPPH free radical and hydroxyl free radical. For the content of total flavones in sweet potato leaves, just a correlation with scavenging rate against hydroxyl-free radical shown in test. [Conclusion] The phenols in ex-tracts could effectively scavenge both the DPPH free radical and hydroxyl free rad/cal, whereas the flavones in extzacts can only function on the hydroxyl free radical.