Immunological analysis of agglutination in Dirofilaria immitis microfllariae |
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Authors: | Hayasaki M |
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Institution: | Veterinary Clinical Center, School of Veterinary Medicine, Yamaguchi University, Japan. |
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Abstract: | The mechanism of agglutination phenomenon of Dirofilaria immitis microfilariae was analyzed. Circulating microfilariae were collected from a D. immitis-infected microfilaremic dog and cultured in the several kinds of sera from dogs and animals. The agglutination of D. immitis microfilariae is a specific phenomenon due to some immune complexes formed with the anti-microfilarial antibody, heat-instable factor(s) and excretory-secretory products of microfilariae. Only live microfilariae were agglutinated and the agglutinated microfilariae remained alive as long as 27 days in culture in vitro. |
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