| 一种适宜拟南芥PCR检测的DNA提取方法 |
| |
| 引用本文： | 徐平丽,赵晋平,孟静静,李保龙,李新国,郭峰. 一种适宜拟南芥PCR检测的DNA提取方法[J]. 安徽农业科学, 2010, 38(13): 6653-6654. DOI: 10.3969/j.issn.0517-6611.2010.13.014 |
| |
| 作者姓名： | 徐平丽 赵晋平 孟静静 李保龙 李新国 郭峰 |
| |
| 作者单位： | 山东省农业科学院高新技术研究中心,山东省作物与畜禽品种改良生物技术重点开放实验室,山东济南,250100;山东省曹县种子公司,山东菏泽,274400 |
| |
| 基金项目： | 国家科技支撑计划，山东省优秀中青年科学家研究奖励基金，山东省农业科学院青年基金 |
| |
| 摘 要： | [目的]介绍一种适于拟南芥PCR检测的DNA快速提取方法。[方法]通过对常规DNA提取方法的改进,获得可以快速大批量地提取拟南芥DNA样品的方法,并以随机抽取的拟南芥转基因株系和突变株系为样品进行验证。[结果]经过琼脂糖凝胶电泳及紫外吸收检测,DNA样品完整且污染少,PCR扩增目的片段结果良好,适于作为PCR反应的模板。经过对随机抽取的拟南芥转基因株系和突变株系的PCR检测,阳性植株目的基因扩增条带清晰,无假阳性,试验结果理想。[结论]该方法适用于拟南芥DNA样品的快速提取、PCR检测及拟南芥突变体筛选工作。
|
| 关 键 词： | 拟南芥 PCR DNA快速提取 |
A Rapid DNA Extraction Method for PCR Detection of Arabidopsis thaliana |
| |
| Affiliation: | XU Ping-li et al ( Key Laboratory of Improved Biotechnology of Crops and Livestock and Poultry in Shandong Province, High-tech Research Center, Shandong Academy of Agricultural Sciences, Jinan, Shandong 250100) |
| |
| Abstract: | [Objective] The aim was to introduce a rapid DNA extraction method for PCR detection of Arabidopsis thaliana. [Method] Through the improvement of conventional DNA extraction method,a rapid Arabidopsis thaliana DNA extraction method was obtained. With randomly selected Arabidopsis thaliana transgenic strains and mutants as samples,the method was verified. [Result] After electrophoresis,UV absorption detection,it was found that DNA samples are complete and less pollution,and the result of PCR amplification objective fragment was good which proved DNA is suitable as a template for PCR reaction. After PCR detection,positive plants gene amplified bands were clear,without false-positive,and the test results were satisfactory. [Conclusion] The method is suitable for rapid extraction of Arabidopsis thaliana DNA、PCR detection and screening of Arabidopsis thaliana. |
| |
| Keywords: | PCR |
| 本文献已被 维普 万方数据 等数据库收录！ |
|
