| 玉米基因枪转化Bt基因的研究 |
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| 引用本文： | 孙红炜,朱常香,杨崇良,尚佑芬,赵玖华,路兴波,王升吉. 玉米基因枪转化Bt基因的研究[J]. 山东农业科学, 2004, 0(3): 20-22 |
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| 作者姓名： | 孙红炜 朱常香 杨崇良 尚佑芬 赵玖华 路兴波 王升吉 |
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| 作者单位： | 1. 山东省农业科学院植物保护研究所,山东,济南,250100
2. 山东农业大学植物保护学院,山东,泰安,271018 |
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| 基金项目： | 本试验在山东农业大学抗病基因工程重点实验室完成. |
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| 摘 要： | 以玉米自交系A188为试材,将含有Bar基因和Bt基因的质粒pBW3导入玉米幼胚内,获得了转基因抗性苗。抗性植株点杂交及Southern杂交结果显示,该基因已整合到抗性玉米植株体内,外源基因在玉米基因组内有4个拷贝。
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| 关 键 词： | 玉米 Bt基因 基因枪 点杂交 Southern杂交 |
| 文章编号： | 1001-4942(2004)03-0020-03 |
| 修稿时间： | 2003-11-06 |
Studies on transformation of Bt.gene into maize by micro- oarticle bombardments |
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| SUN Hong-wei,ZHU Chang-xiang,YANG Chong-liang,SHANG You-fen,ZHAO Jiu-hua,LU Xing-bo,WANG Sheng-ji. Studies on transformation of Bt.gene into maize by micro- oarticle bombardments[J]. Shandong Agricultural Sciences, 2004, 0(3): 20-22 |
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| Authors: | SUN Hong-wei ZHU Chang-xiang YANG Chong-liang SHANG You-fen ZHAO Jiu-hua LU Xing-bo WANG Sheng-ji |
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| Affiliation: | SUN Hong-wei~1,ZHU Chang-xiang~2,YANG Chong-liang~1,SHANG You-fen~1,ZHAO Jiu-hua~1,LU Xing-bo~1,WANG Sheng-ji~1 |
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| Abstract: | By micro-particle bombardments, Plasmid pBW3 which is component of Bt.and Bar gene was transferred into maize immature embryos of maize self-bred lines A188, resistant maize plants were obtained.Dot-bloting and Southern-bloting of transgenic maize leaves showed:Bt.gene had integrated into resistant maize plants, heterogeneous gene had four copies in maize gene group. |
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| Keywords: | Maize Bt.gene micro-particle gun dot-bloting Southern-bloting |
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