碱性溶液提取荞麦水溶性多糖的研究

研究用碱溶液（0．3mol／LNaOH）提取养麦水溶性多糖，分别用料液比（1：30，1：40，1：50）和提取时间（1h，2h，3h），提取温度（70℃，80℃，90℃）3因素3水平正交法提取多糖，并研究了这3个因素对多糖得率的影响，方差分析表明温度对多糖提取影响较显著（P〈0．1），料液比和时间影响不显著。在本实验条件下，荞麦多糖的得率迭25．16％。同时用Sevag方法除蛋白，石油醚回流脱脂，淀粉酶，纤维素酶除去淀粉和纤维素，双氧水脱去色素对多糖进行了纯化。

Isolation of Buckwheat Polysaccharide by Alkali method

This study was on extracting polysaecharide from buckwheat by orthogonal design. Selected three factors （Solid- solvent ratio： 1 ： 30,1 ： 40,1 ： 50; extracting temperature： 70℃,80℃ ,90℃ and extracting time： lh,2h, 3h）. The results showed extracting temperature was significant （P〈0. 1） to extract buckwheat polysaccharide , then Solid-solvent ratio and extracting time were not significant（P〉0. 1）. under this condition, polysaccharide extracting rate was 25.16%. Meanwhile, removed protein using Sevag method, removed oil using petroleum ether , removed amylum and cellulose using amylase and cellulase, removed pigment using H2O2 to purify polysaccharide.