mtDNA D-loop of Chinese main indigenous sheep breeds using PCR-RFLP |
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| Authors: | Xianglong Li Zengli Zhang Yuanfang Gong Zhengzhu Liu Qing Jia Lize Wang |
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| Affiliation: | (1) Department of Animal Science & Technology, Agricultural University of Hebei, Baoding, 071001, China;(2) Department of Animal Science Hebei Normal University of Science & Technology, Changli, 066600, China |
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| Abstract: | The polymorphism of mitochondrial DNA (mtD-NA) D-loop of 83 individuals from nine Chinese indigenous sheep breeds and two imported sheep breeds was studied with five endonucleases, Hinf I, Msp I, Sau3 A I, Xsp I and Taq I, using PCR-RFLP. The results indicated that there existed two basic haplotypes in the region of mtDNA D-loop. It could be inferred that Chinese indigenous sheep breeds originated from two maternal ancestors. The average polymorphic degree (π value = 0.0421%) of mtDNA D-loop showed that the genetic diversity of mtDNA of Chinese indigenous sheep breeds was very low. Translated from Hereditas, 2006, 28(2): 165–170 [译自: 遗传] |
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| Keywords: | indigenous sheep breeds mtDNA D-loop PCR-RFLP |
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