无花果多糖提取工艺及其功能研究 |
| |
引用本文: | 叶文斌. 无花果多糖提取工艺及其功能研究[J]. 甘肃农业大学学报, 2016, 0(3): 140-149. DOI: 10.3969/j.issn.1003-4315.2016.03.023 |
| |
作者姓名: | 叶文斌 |
| |
作者单位: | 陇南师范高等专科学校农林技术学院,甘肃 成县,742500 |
| |
基金项目: | 甘肃省教育厅资助项目(0928B-1);甘肃省自然科学基金项目(1107RJZK243);陇南师范高等专科学校重点项目(2014LSZK01001). |
| |
摘 要: | 【目的】优化无花果多糖提取工艺,研究其生物功能.【方法】采用响应面与酶法联用优化无花果(Ficus carica)多糖的提取工艺,测定无花果多糖对糖尿病诱导大鼠血脂代谢指标的影响.【结果】无花果多糖提取最佳优化条件为酶解温度50.36℃,酶用量0.06g和pH 4.73.该条件下无花果多糖提取预测值为34.47%,验证值为34.13%.用无花果多糖连续给大鼠灌胃给药28d,发现无花果多糖能显著降低正常大鼠血糖(P0.05),极显著降低肾上腺素引起的高血糖(P0.01),有效降低糖尿病大鼠的FBG、HbAlc、TC、TG,升高HDL含量.【结论】获得了无花果多糖提取最佳工艺参数,无花果多糖能够显著降低糖尿病大鼠血糖,并显著改善糖尿病大鼠血脂代谢紊乱.
|
关 键 词: | 效应面法 酶法 无花果多糖 优化工艺 四氧嘧啶诱导糖尿病大鼠 |
Optimization of polysaccharide extraction process from Ficus carica and its function |
| |
Abstract: | Obj ective]To optimize the technology of polysaccharides extration from Ficus carica,and study its health to alloxan-induced diabetic rats function.[Method]Extracting process of polysaccharides from Ficus carica were optimized by response surface methodology and enzymolysis,and investigating the effects of polysaccharides on glucose and lipidemia and acute toxicity in alloxan-induced diabetic rats.[Re-sult]The optimal extraction conditions were hydrolysis temperature of 50.36 ℃,hydrolysis concentration of 0.06 g ,extraction pH of 4.73 and extracting three times.Under the conditions,the predicted and proved value of polysaccharides extracted were 34.47%and 34.13%,respectively.If polysaccharides were contin-uously intragastric administration for 28 days ,the polysaccharides could significantly reduce blood glucose adrenaline induced hyperglycemia in normal rats and could decrease the levels of FBG ,HbAlc,TC and TG in serum in alloxan-induced diabetic rats,and increase the concentrations of HDL significantly.[Conclu-sion]The optimal process parameters for extracting polysaccharides from Ficus carica was obtained ,and the polysaccharides could significantly decrease the blood glucose and improve the disorder of lipid metabo-lism in alloxan-induced diabetic rats. |
| |
Keywords: | response surface methodology enzymolysis Ficus carica polysaccharides optimization process alloxan-induced diabetic rat |
本文献已被 CNKI 万方数据 等数据库收录! |
|