| 副猪嗜血杆菌PCR检测方法的建立与初步应用 |
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| 引用本文： | 万世平,王建,葛菲菲,徐峰,沈丽萍,刘佩红,费荣梅. 副猪嗜血杆菌PCR检测方法的建立与初步应用[J]. 动物医学进展, 2009, 30(1) |
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| 作者姓名： | 万世平 王建 葛菲菲 徐峰 沈丽萍 刘佩红 费荣梅 |
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| 作者单位： | 南京农业大学农业部动物疫病诊断与免疫重点开放实验室,江苏,南京,210095;上海市动物疫病预防控制中心,上海,201103 |
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| 基金项目： | 上海市科委重大攻关项目 |
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| 摘 要： | 根据副猪嗜血杆菌16 S rRNA基因设计了一对引物,通过最佳条件摸索扩增出大小为821 bp的特异目的基因片段,建立了快速检测副猪嗜血杆茵的PCR方法,该方法最低检出量达10-3 ng,且对大肠埃希茵、金黄色葡萄球菌、传染性胸膜肺炎放线杆菌和巴氏杆茵等均无交叉反应.用该PCR方法从门诊送检的病料中检测出4株副猪嗜血杆菌,并对分离株SH0854P的PCR扩增产物进行测序与对比分析,其与已发表的GenBank中的相关菌株的同源性为97.3%～100%.
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| 关 键 词： | 副猪嗜血杆菌 PCR 测序 |
Development and Clinical Application of a PCR Assay for Detection of Haemophilus parasuis |
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| WAN Shi-ping,WANG Jian,GE Fei-fei,XU Feng,SHEN Li-ping,LIU Pei-hong,FEI Rong-mei. Development and Clinical Application of a PCR Assay for Detection of Haemophilus parasuis[J]. Progress In Veterinary Medicine, 2009, 30(1) |
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| Authors: | WAN Shi-ping WANG Jian GE Fei-fei XU Feng SHEN Li-ping LIU Pei-hong FEI Rong-mei |
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| Affiliation: | 1.Key Laboratory of Animal Disease Diagnosis and Immunology;Ministry of Agriculture at Nanjing Agricultural University;Nanjing;Jiangsu;210095;China;2.Shanghai Animal Disease Control Center;Shanghai;201103;China |
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| Abstract: | The PCR assay was established for the rapid detection of Haemophilus parasuis with a pair of primers designed according to the specific conserved sequence of 16 S rRNA gene.The amplified product was a fragment with 821 bp in length by groping the optimum conditions.Simultaneously,specificity and sensitivity assays revealed that there is no cross reaction with Escherichia coli,Staphylococcus,Actinobacillus pleuropneumoniae and Pasteurella.it is specific to Haemophilus parasuis with a minimum detection sensit... |
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| Keywords: | PCR |
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