| 非洲菊的离体培养研究 |
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| 引用本文： | 杨梦玲,杨梦湘,丁江南,朱丽娜.非洲菊的离体培养研究[J].湖南农业科学,2009(4). |
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| 作者姓名： | 杨梦玲 杨梦湘 丁江南 朱丽娜 |
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| 作者单位： | 湖南农业大学园艺园林学院,湖南,长沙,410128;湖南农业大学后勤服务集团,湖南,长沙,410128 |
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| 摘 要： | 以非洲菊的侧芽为试材,在MS培养基中添加不同种类与不同浓度的生长调节剂进行离体培养。研究结果表明,以MS＋2.0 mg/L 6-BA＋0.3 mg/L IBA＋30 g/L蔗糖＋7 g/L琼脂的固体培养基为诱导不定芽的最佳培养基;以MS＋1.5 mg/L IAA和1/2MS＋0.03 mg/L NAA＋30 g/L蔗糖＋7 g/L琼脂的固体培养基诱导生根效果好;幼苗移栽的基质以白砂土（已灭菌）＋20%已发酵的棉籽壳（体积比）为最佳,成活率可达95%。该项研究对非洲菊的快速繁殖有一定的指导作用。
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| 关 键 词： | 非洲菊 离体 生长调节剂 培养基 |
Study on Tissue Culture of Gerbera Jamesonii Blous.G Hybrida Hort |
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| YANG Meng-ling,YANG Meng-xiang,DING Oiang-nan,ZHU Li-na.Study on Tissue Culture of Gerbera Jamesonii Blous.G Hybrida Hort[J].Hunan Agricultural Sciences,2009(4). |
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| Authors: | YANG Meng-ling YANG Meng-xiang DING Oiang-nan ZHU Li-na |
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| Institution: | 1.College of Horticulture and Landscape Experimental Teaching Center;HNAU;Changsha 410128;PRC;2.Logistics Services Group;PRC |
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| Abstract: | Using suckers of Gerbera Jamesonii Blous.G hybrida Hort as materials,different concentrations of different kinds of plant growth regulator were added in MS culture in vitro culture.The result indicated that the MS medium containing 2.0 mg/L 6-BA,0.3mg/L IBA,30 g/L sucrose and 7.0 g/L sugar was the best medium for bringing out the adventitious bud;MS containing 1.5mg/L IAA and 1/2MS containing 0.03mg/L NAA,30 g/L sucrose and 7.0 g/L sugar had good effect on bringing out root;the medium composed of sterilized... |
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| Keywords: | Gerbera Jamesonii Blous G hybrida Hort exlianting growth hormone medium |
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