| 新疆绵羊种布鲁氏菌OMP25基因的分子克隆及核苷酸序列的测定 |
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| 引用本文： | 柳建新,陈创夫,田晶华.新疆绵羊种布鲁氏菌OMP25基因的分子克隆及核苷酸序列的测定[J].中国兽医杂志,2005,41(11):6-8. |
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| 作者姓名： | 柳建新 陈创夫 田晶华 |
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| 作者单位： | 石河子大学动物科技学院新疆地方与民族高发病重点实验室,新疆,石河子,832003 |
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| 摘 要： | 根据GeneBank库上国际标准菌株绵羊种布鲁氏菌（63／290）的OMP25的基因序列设计引物，用聚合酶链式反应（PcR）技术从新疆绵羊种布鲁氏菌（80／019）基因组DNA中扩增出OMP25基因片段，TtDNA连接酶将其连接于PBS-T克隆载体质粒上，将重组质粒转化到受体菌DH10B中，蓝白斑筛选阳性菌落，结果成功克隆OMP25基因片段，进行核苷酸序列测定，测序结果分析表明：新疆绵羊菌株与国际标准菌株有明显差异。
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| 关 键 词： | 新疆绵羊种布鲁氏菌 OMP25 克隆 核苷酸序列分析 |
| 文章编号： | 0529-6005（2005）11-0006-03 |
| 收稿时间： | 2004-11-04 |
| 修稿时间： | 2004年11月4日 |
Cloning and sequence analysis of the OMP25 gene of Brucella ovis in Xinjiang sheep |
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| LIU Jian-xin,CHEN Chuang-fu,TIAN Jing-hua.Cloning and sequence analysis of the OMP25 gene of Brucella ovis in Xinjiang sheep[J].Chinese Journal of Veterinary Medicine,2005,41(11):6-8. |
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| Authors: | LIU Jian-xin CHEN Chuang-fu TIAN Jing-hua |
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| Institution: | Key Laboratory of Xinjiang Endemic and Ethnic Diseases, Animal Science Technology College, Shihezi University 832003 |
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| Abstract: | Primers were devised according to putative omp25 gene sequence which was named as international standard strand 63/290, B. ovis. We took Xinjiang local B. ovis 80/019 as template, amplified gene fragment by PCR, ligated the fragment with PBS-T by T4 DNA ligase, transformed recombinant plasmid to E. coli DH10B by heat shock. The transformants were screened on ampicillin/Xgal-IPTG, on plate. Result- successfully cloned OMP25 gene fragment . Analysis of OMP25 gene sequence revealed that it was obviously different in OMP25 gene sequence of Xinjiang local B. ovis and that of international standard strain. |
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| Keywords: | Xinjiang Brucella ovis OMP25 gene cloning |
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