|
|||||
|
|
| 纳豆激酶植物高效表达载体的构建 | |
| 引用本文： | 张锋,金杰,解成骏,谭振波,杨公明.纳豆激酶植物高效表达载体的构建[J].西北农业学报,2005,14(3):159-162. |
| 作者姓名： | 张锋 金杰 解成骏 谭振波 杨公明 |
| 作者单位： | 1. 西北农林科技大学食品科学与工程学院,陕西杨凌,712100;北京市农林科学院北京农业生物技术研究中心,北京,100089 2. 西北农林科技大学食品科学与工程学院,陕西杨凌,712100 3. 云南文山师范高等专科学院,文山,663000 4. 北京市农林科学院北京农业生物技术研究中心,北京,100089 5. 西北农林科技大学食品科学与工程学院,陕西杨凌,712100;华南农业大学食品学院,广州,510642 |
| 摘 要： | 纳豆激酶来源于日本的传统食品—纳豆,是一种具有强烈溶栓作用的蛋白激酶,将编码纳豆激酶的基因克隆到含C aM V 35S控制下的双元表达载体pCAM B IA 1300中,PCR结果表明,纳豆激酶基因正向插入到载体中,再通过冻融法将其导入根癌农杆菌LBA 4404中,从而成功地构建了能在植物中高效表达的植物载体,为用植物作为生物反应器来获取纳豆激酶蛋白提供了一条新途径。 |
| 关 键 词： | 植物生物反应器 纳豆激酶 表达载体 农杆菌 |
| 文章编号： | 1004-1389（2005）03-0159-04 |
| 收稿时间： | 2005-01-10 |
| 修稿时间： | 2005年1月10日 |
Construction of Plant Efficient Expression Vector on Nattokinase |
|
| ZHANG Feng,JIN Jie,XIE Cheng-jun,TAN Zhen-bo and YANG Gong-ming.Construction of Plant Efficient Expression Vector on Nattokinase[J].Acta Agriculturae Boreali-occidentalis Sinica,2005,14(3):159-162. | |
| Authors: | ZHANG Feng JIN Jie XIE Cheng-jun TAN Zhen-bo and YANG Gong-ming |
| Institution: | College of Food Science and Engineering, Northwest A & F University, Yangling Shaanxi 712100, China;Beijing Agro-biotechnology Research Center, Beijing Academy of Agricultural & Forestry Science, Beijing 100089, China;College of Food Science and Engineering, Northwest A & F University, Yangling Shaanxi 712100, China;Whenshan Teachers'' College, Whenshan 663000, China;Beijing Agro-biotechnology Research Center, Beijing Academy of Agricultural & Forestry Science, Beijing 100089, China;College of Food Science and Engineering, Northwest A & F University, Yangling Shaanxi 712100, China;College of Food, South China Agricultural University, Guangzhou 510642, China |
| Abstract: | Nattokinase,extracted from the Japanese food-natto, is found to be a strong fibrinolytic enzyme.The gene encoded Nattokinase was cloned into expressing vector-pCAMBIA1300 including CaMV35S promoter,PCR result indicated that NK gene was positively inserted,then the expression vector was transferred into Agrobacterium tumefaciens LBA4404 using the freeze-thaw method.Therefore,the vector,which could be efficiently expressed in plants,was successfully constructed,this can provide a new approach of gaining nattokinase protein by using plants as bioreactors. |
| Keywords: | Plant bioreactors Nattokinase Expression vector Agrobacterium tumefaciens |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |
| 点击此处可从《西北农业学报》浏览原始摘要信息 | |
| 点击此处可从《西北农业学报》下载免费的PDF全文 | |