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| 结球甘蓝外源基因转化再生时的激素条件研究 | |
| 引用本文： | 王晓峰,鲁瑞芳,彭学贤. 结球甘蓝外源基因转化再生时的激素条件研究[J]. 西北农林科技大学学报(自然科学版), 2000, 28(1): 43-47 |
| 作者姓名： | 王晓峰 鲁瑞芳 彭学贤 |
| 作者单位： | 1. 中国科学院微生物研究所,北京,100080;西北农业大学园艺系,陕西杨陵,712100 2. 中国科学院微生物研究所,北京,100080 3. 西北农业大学园艺系,陕西杨陵,712100 |
| 基金项目： | ：国家自然科学基金资助项目(39670413) |
| 摘 要： | 结球甘蓝(Brassicaoleracevar.capitataL.)转化时,子叶柄、下胚轴受农杆菌(Agrobacteriumtumefaciens)侵染后,芽再生的激素配比研究表明,中甘8号在激素配比为BA4.0mg·L-1+IAA(1.0～2.0)mg·L-1时,子叶柄和下胚轴芽的诱导率为100%;在BA1.0mg·L-1+ZT1.0mg·L-1+NAA(0.10～0.15)mg·L-1时,子叶柄芽的诱导率为60%左右;在BA1.0mg·L-1+ZT1.0mg·L-1+NAA(0.15～0.20)mg·L-1时,下胚轴芽的诱导率为100%。激素配比与品种间互作效应小,以因素主效应为主。与未侵染外植体芽诱导比较,认为结球甘蓝受农杆菌侵染后,离体芽诱导需要较高的生长素质量浓度。 |
| 关 键 词： | 结球甘蓝 遗传转化 芽再生 |
| 文章编号： | 1000-2782(2000)01-0043-05 |
| 修稿时间： | 1999-02-25 |
Studies on hormonal combinations of regeneration in vitro in cabbage (Brassica olerace var.capitata L.) transformation |
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| WANG Xiao-feng. Studies on hormonal combinations of regeneration in vitro in cabbage (Brassica olerace var.capitata L.) transformation[J]. Journal of Northwest A&F University（Natural Science Edition）, 2000, 28(1): 43-47 | |
| Authors: | WANG Xiao-feng |
| Affiliation: | 1 Institute of Microbiology,Chinese Academy of Sciences,Beijing 100080,China)(2 Department of Horticulture,Northwestern Agricultural University,Yangling,Shaanxi 712100,China) |
| Abstract: | The cotyledonary petioles and hypocotyles of cabbage in vitro infected by Agrobacterium tumefaciens regenerated in hormonal combinations on cabbage (Brassica olerace var.capitata L.)were studied.The results showed that the shoot induction rate of cotyledonary petioles and hypocotyleswas 100% with BA 4.0mg·L-1+ IAA 1.0-2.0mg·L-1 on cabbage cultivarZhonggan No.8; about 60% with BA 1.0mg·L-1+ZT 1.0mg·L-1+NAA 0.10-0.15mg·L-1; 100% with BA 1.0mg·L-1+ZT 1.0mg·L-1+NAA (0.15-0.20)mg·L-1.The interaction of hormonal combinitions and cultivarswas smaller than themain effects of them.Compared with uninfected explants by A.tumefaciens, it is considered that the optimal hormonal combinations of shoot induction contained higher auxin concentration on explants in vitro infected by A.tumefaciens. |
| Keywords: | Brassica olerace var. capitata L. genetic transformation shoot regeneration |
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