| 菊花叶片离体高效再生体系的建立 |
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| 引用本文： | 刘军,赵兰勇,丰震,张美蓉,韩进,杨传强.菊花叶片离体高效再生体系的建立[J].山东农业大学学报(自然科学版),2004,35(2):177-182. |
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| 作者姓名： | 刘军 赵兰勇 丰震 张美蓉 韩进 杨传强 |
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| 作者单位： | 山东农业大学,林学院,山东,泰安,271018 |
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| 摘 要： | 本文报道了以菊花叶片为外植体，诱导植株再生，建立高效植株再生体系。获得诱导愈伤组织、芽和根的最佳培养基。在有6-BA、NAA的MS培养基上获得较高的愈伤组织诱导率，在有2，4-D的培养基上其愈伤组织诱导率很低。带有愈伤组织的外植体转移到有6-BA、NAA的MS培养基上催化芽的产生，并筛选出能直接诱导芽再生的培养基(MS 6-BA(3mg／L) NAA(1mg／L))。AgNO3可明显提高芽的再生率。茎段在1／2MS和有IBA或NAA的1／2MS培养基上诱导生根。生根的小苗在温室里生长良好。
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| 关 键 词： | 菊花 叶片离体 再生体系 外植体 组织培养 培养基 愈伤组织 |
| 文章编号： | 1000-2324(2004)02-0177-06 |
| 修稿时间： | 2003年10月13 |
ESTABALISHMENT OF HIGH EFFICIENT REGENERATION SYSTEM FROM LEAVES OF CHRYSANTHEMUN |
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| LIU Jun,ZHAO Lan-yong,FENG Zhen ,ZHANG Mei-rong,HAN Jin,YANG Chuan-qiang.ESTABALISHMENT OF HIGH EFFICIENT REGENERATION SYSTEM FROM LEAVES OF CHRYSANTHEMUN[J].Journal of Shandong Agricultural University,2004,35(2):177-182. |
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| Authors: | LIU Jun ZHAO Lan-yong FENG Zhen ZHANG Mei-rong HAN Jin YANG Chuan-qiang |
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| Institution: | LIU Jun,ZHAO Lan-yong,FENG Zhen *,ZHANG Mei-rong,HAN Jin,YANG Chuan-qiang |
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| Abstract: | A regeneration system from leaves of chrysanthemum (Dendranthema morifolium) was developed. The optimum media were obtained for callus, shoot regeneration and shoot rooting. On the media with 6-BA, NAA and 2,4-D, calli were induced from leaf explants. After explants with calli were transferred onto MS media with 6-BA and NAA to trigger shoot regeneration, adventitious shoots were regenerated from leaf explants of chrysanthemum, and the medium(MS+6-BA(3mg/L)+NAA(1mg/L)), which could directly induce shoot regeneration, was selected. AgNO3 enhanced shoot induction rate. Shoots were rooted on half-strength MS media with or without IBA or NAA. The rooted plantlets survived in greenhouse. |
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| Keywords: | chrysanthemum leaf disc regeneration system |
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