| 峨眉拟单性木兰多酚氧化酶活性与总酚含量的初步研究 |
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| 引用本文： | 马明东,陈英,易同培,刘跃建,杨林.峨眉拟单性木兰多酚氧化酶活性与总酚含量的初步研究[J].福建林业科技,2009,36(3). |
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| 作者姓名： | 马明东 陈英 易同培 刘跃建 杨林 |
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| 作者单位： | 四川农业大学都江堰分校,四川都江堰,611830 |
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| 基金项目： | 世界自然基金会(WWF)物种保护项目"峨眉拟单性木兰物种保护" |
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| 摘 要： | 以峨眉拟单性木兰嫩枝茎段为测试材料,研究多酚氧化酶(PPO)活性和总酚含量及其季节变化规律。结果表明,测定峨眉拟单性木兰PPO活性的最适pH值为7.0,最佳底物为0.14 mol.L-1的邻苯二酚,最佳温度为25℃;峨眉拟单性木兰PPO活性在生长期明显高于休眠期;峨眉拟单性木兰总酚含量在每年的4月、5月、9月3个时段最低,为无性繁殖取材最佳时间。
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| 关 键 词： | 峨眉拟单性木兰 多酚氧化酶活性 总酚含量 |
Preliminary Study on Polyphenol Oxidase(PPO) Activity and Total Phenol Content of Parakmeria omeiensis Cheng |
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| MA Ming-dong,CHEN Ying,YI Tong-pei,LIU Yue-jian,YANG Lin.Preliminary Study on Polyphenol Oxidase(PPO) Activity and Total Phenol Content of Parakmeria omeiensis Cheng[J].Journal of Fujian Forestry Science and Technology,2009,36(3). |
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| Authors: | MA Ming-dong CHEN Ying YI Tong-pei LIU Yue-jian YANG Lin |
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| Institution: | MA Ming-dong,CHEN Ying,YI Tong-pei,LIU Yue-jian,YANG Lin(Sichuan University of Agriculture,Dujiangyan 611830,Sichuan,China) |
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| Abstract: | Taking burgeon and stem of Parakmeria omeiensis Cheng as test materials,polyphenol oxidase(PPO),total phenol content,and seasonal change law were studied.The results showed the suitable pH value for determining P.omeiensis PPO activit was 7.0,the optimal substrate was 0.14 mol·L-1 of oxophenic acid,the optimal temperature was 25℃;P.omeiensis PPO enzyme activity at the growth stage was higher than dormancy stage,P.omeiensis total phenol content was lowest in Apil,May,and September in a year,and which was the optimal time for getting the cloning stuffs. |
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| Keywords: | Parakmeria omeiensis Cheng polyphenol oxidase activity total phenol content |
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