大鲵精液离体保存的初步研究

针对大鲵(Andrias davidianus)的性腺发育不同步，为提高受精率，对其精液特性和保存条件进行初步研究。人工辅助采集精液，对精液量、酸碱度、密度、活力等基本特性进行常规测定。分别对保护液、光照、温度等保存条件进行分组研究试验。结果表明：雄性亲鲵的精液量最大可达12 ml/kg，pH值6.7～7.0微酸状态，密度215～1075万/ml，精子活力为0.7～0.9。离体保存条件下，室温暗环境（20LUX）较光环境（400LUX）可延长存活时间0.5h，暗环境低温（0～4℃）较室温（21～22℃）可延长存活时间3d，低温暗环境原精液中添加保护液比对照组精子存活时间延长6～12h。

Preliminary Study on Conservation of Andrias davidanus Semen in Vitro

Gonadal development of Andrias davidanus not synchronized,semen characteristics and preservation conditions of A.davidanus were studied targeting the increase of fertility rate.Semina were collected by artificial assistance approach,and a regular assay was conducted on the basic features such as semen volume,pH,density,vitality,etc.Preservation conditions,namely,protection medium,illumination and temperature,were also studied.The results showed that semen volume of pro-male A.davidanus was up to 12 mL/kg,pH 6.7-7.0 of subacid condition;and that the semen density was(2.15-10.75)million/mL,sperm motility 0.7-0.9.In vitro conservation conditions,dark environment at ambient illuminance(20 lx).The result also indicated that the survival time of the semina was 0.5 h longer than those conserved in the luminous environment(400 lx);low temperature(0-4℃) could extend the survival time of the semina 3d longer than those at room temperature(21-22℃) in dark environment;in the cold dark environment,semina with protection medium added in could extend the survival time 6-12 h longer than the control group.