Screening Method for Transgenic Maize with Kanamycin Resistance |
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Authors: | LU Meng-yu DONG Fu-shuang ZHANG Jun-min WANG Hai-bo Hebei Center of Plant Genetic Transformation |
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Affiliation: | LU Meng-yu,DONG Fu-shuang,ZHANG Jun-min,WANG Hai-bo* Hebei Center of Plant Genetic Transformation,Institute of Genetic and Physiology,Hebei Academy of Agricultural and Forestry Sciences,Shijiazhuang 050051 |
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Abstract: | [Objective] The paper was to establish simple and effective method to screen marker gene in maize with kanamycin resistance.[Method] Using inbred line Chang 7-2 and hybrid Zhengdan 958 of maize as test materials,their seeds were soaked with different concentrations and volumes of kanamycin for 3 and 4 d,respectively,the rate of albino seedlings and average seedling height after sowing for 10 d were investigated.[Result] The rate of albino seedlings not only was related to kanamycin concentration,but also ha... |
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Keywords: | Transgenic maize Kanamycin Screening method |
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