| Asia I口蹄疫病毒组合基因P1-2A3C在不同家蚕品种中的表达(英文) |
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| 引用本文： | 张韵,张金卫,易咏竹,李志勇,李江涛,鱼南洋,丁农,柳纪省. Asia I口蹄疫病毒组合基因P1-2A3C在不同家蚕品种中的表达(英文)[J]. （《农业科学与技术》）编辑部, 2011, 0(10) |
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| 作者姓名： | 张韵 张金卫 易咏竹 李志勇 李江涛 鱼南洋 丁农 柳纪省 |
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| 作者单位： | 中国农业科学院兰州兽医研究所家畜疫病病原生物学国家重点实验室农业部畜禽病毒学重点开放实验室;湖州市农业科学研究院;中国农科院生物技术所; |
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| 基金项目： | Supported by863Project for Development and Production of New FMDV accine for Livestock(2011AA10A211); Fund for Agricultural Since and Technology Achievements Transformation(2009GB23260437)~~ |
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| 摘 要： | [目的]研究AsiaI口蹄疫病毒组合基因P1-2A3C在不同家蚕品种中的表达,以期筛选出较适合表达该组合基因的家蚕品种。[方法]将已经获得高效表达的重组家蚕杆状病毒rBmNPV(P1-2A3C)基因,分别注射原种及杂交家蚕蚕蛹。利用ELISA方法对其进行抗原表达量检测,并对表达结果进行差异比较。[结果]不同家蚕品种对rBmNPV(P1-2A3C)的表达存在着明显差异;秋丰×TQ78和秋丰×丝胶茧杂交组合可考虑作为高效表达的家蚕生物反应器专用品种。[结论]为AsiaIFMDV目的蛋白的高效表达专用品种的选育提供了依据。
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| 关 键 词： | AsiaI口蹄疫病毒 P1-2A3C 家蚕品种 |
Expression of Recombinant Gene P1-2A3C of FMDV Asia I in Different Bombyx mori Varieties |
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| ZHANG Yun,ZHANG Jin-wei,YI Yong-zhu,LI Zhi-yong,LI Jiang-tao,YU Nan-yang,DING Nong,LIU Ji-xing .Key Laboratory of Animal Virology of Ministry of Agriculture,State Key Laboratory of Veterinary Etiological Biology,Lanzhou Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Lanzhou ,.Huzhou Academy of Agricultural Sciences,Huzhou ,.Biotechnology Research Institute,Beijing. Expression of Recombinant Gene P1-2A3C of FMDV Asia I in Different Bombyx mori Varieties[J]. 农业科学与技术(英文版)Agricultural Science ＆ Technology, 2011, 0(10) |
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| Authors: | ZHANG Yun ZHANG Jin-wei YI Yong-zhu LI Zhi-yong LI Jiang-tao YU Nan-yang DING Nong LIU Ji-xing .Key Laboratory of Animal Virology of Ministry of Agriculture State Key Laboratory of Veterinary Etiological Biology Lanzhou Veterinary Research Institute Chinese Academy of Agricultural Sciences Lanzhou .Huzhou Academy of Agricultural Sciences Huzhou .Biotechnology Research Institute Beijing |
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| Affiliation: | ZHANG Yun~1*,ZHANG Jin-wei~2*,YI Yong-zhu~3,LI Zhi-yong~1,LI Jiang-tao~2,YU Nan-yang~2,DING Nong~1,LIU Ji-xing~1** 1.Key Laboratory of Animal Virology of Ministry of Agriculture,State Key Laboratory of Veterinary Etiological Biology,Lanzhou Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Lanzhou 730046,2.Huzhou Academy of Agricultural Sciences,Huzhou 313000,3.Biotechnology Research Institute,Beijing 100081 |
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| Abstract: | [Objective] The aim was to investigate the expression level of recombinant gene P1-2A3C of FMDV Asia I in different Bombyx mori varieties,so as to screen out the B.mori varieties suitable for the foreign gene expression.[Method] The recombinant B.mori baculovirus rBmNPV(P1-2A3C) are injected into pupae of original species and hybrids of B.mori respectively.Then,the expression of antigen is detected by ELISA method and compared.[Result] The expression level of P1-2A3C significantly differed in different B.mo... |
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| Keywords: | Asia I FMDV P1-2A3C Bombyx mori varieties |
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