| 干旱和盐碱共胁迫玉米EST数据库分析(英文) |
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| 引用本文： | 豆长明,张洁.干旱和盐碱共胁迫玉米EST数据库分析(英文)[J].Agricultural Science&Technology,2011(7). |
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| 作者姓名： | 豆长明 张洁 |
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| 作者单位： | 安徽省环境科学研究院; |
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| 摘 要： | 目的]构建干旱和盐碱共胁迫的玉米cDNA文库,对细胞功能相关基因表达进行初步分析。方法]提取处理玉米YQ7-96品系的雌雄分化期(12片叶龄)的混合组织(叶、茎和花芽)总RNA,运用SMART技术构建pDNR-LIB载体的cDNA文库,并随机挑选克隆进行EST序列的BlastX比对和MIPS归类分析。结果]随机挑取3 027个cDNA克隆进行测序,94.45%的EST长度大于400 bp,共获得1 861个单基因簇,其中维持正常生理活动的基因占65.36%;参与细胞内运输、细胞内信号传导、细胞防御和细胞循环和DNA代谢过程的基因分别占9.26%、6.58%、2.63%和3.16%。结论]在成功构建干旱和盐碱共胁迫的玉米cDNA文库的基础上,对产生的EST进行了测序和分析归类,筛选出了细胞功能相关EST,为后续研究奠定了基础。
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| 关 键 词： | 玉米 干旱 盐碱 共胁迫 EST |
Analysis on Maize EST Database under the Co-stress of Drought,Salt and Alkali |
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| DOU Chang-ming,ZHANG Jie Anhui Academy of Environmental Sciences,Hefei.Analysis on Maize EST Database under the Co-stress of Drought,Salt and Alkali[J].农业科学与技术(英文版),2011(7). |
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| Authors: | DOU Chang-ming ZHANG Jie Anhui Academy of Environmental Sciences Hefei |
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| Institution: | DOU Chang-ming*,ZHANG Jie Anhui Academy of Environmental Sciences,Hefei 230022 |
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| Abstract: | Objective] The research aimed to construct maize cDNA library under the co-stress of drought,salt and alkali,and initially analyze the expression of gene which related to the cellular function.Method] The total RNA of mixed tissue(leaf,stem and blossom bud) of maize YQ7-96 in the male and female differentiation period(12-leaf age) was extracted.By using SMART technology,cDNA library of pDNR-LIB vector was constructed.BlastX contrast and MIPS classification analysis of EST sequence were carried out by rand... |
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| Keywords: | Maize Drought Salt and alkali Co-stress EST |
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