| 一株拮抗棉花枯萎病菌产铁载体内生细菌的筛选(英文) |
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| 引用本文： | 韩松,张守村,林天兴,龚明福. 一株拮抗棉花枯萎病菌产铁载体内生细菌的筛选(英文)[J]. （《农业科学与技术》）编辑部, 2011, 0(7) |
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| 作者姓名： | 韩松 张守村 林天兴 龚明福 |
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| 作者单位： | 塔里木盆地生物资源保护利用兵团重点实验室塔里木大学生命科学学院;乐山师范学院化学与生命学院; |
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| 基金项目： | Supported by National Natural Science Foundation of China(30960010 ); Principal Fund Key Projects of Tarim University(TDZKZD06001)~~ |
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| 摘 要： | [目的]以盆栽控病效果良好的一株植物内生细菌为材料,通过对铁载体的研究,探索其对棉花枯萎病的拮抗机制。[方法]通过蔗糖-天冬氨酸(Modified Sugar-Aspartic Acid,MSA)平板初步判断菌株是否产铁载体及其荧光特性;根据特定波长下吸光度值测定各菌株在液体MSA培养基内产铁载体活性;不同铁离子浓度下,比较其铁载体对棉花枯萎病的抑制作用;结合形态、生理生化等特征对其进行初步鉴定。[结果]该内生细菌在MSA培养基中高产的荧光铁载体对棉花枯萎病原真菌——尖孢镰刀菌(Fusarium oxysporum)具有抑制作用,随着铁离子浓度的提高对病原真菌的抑制作用减弱;初步分析确定该内生菌株属于芽孢杆菌属(Bacillus)。[结论]芽孢杆菌可以通过分泌铁载体竞争铁的吸收而抑制尖孢镰刀菌的生长。
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| 关 键 词： | 内生细菌 铁载体 棉花枯萎病菌 拮抗 |
Screening for Siderophore-producing Endophytic Bacteria against Fusarium oxysporum |
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| HAN Song,ZHANG Shou-cun,LIN Tian-xing,GONG Ming-fu, .Key Laboratory of Protection , Utilization of Biological Resources in Tarim Basin of Xinjiang Production Construction Corps,College of Life Science,Tarim University,Alar ,.School of Chemistry , Life Sciences,Leshan Normal University,Leshan. Screening for Siderophore-producing Endophytic Bacteria against Fusarium oxysporum[J]. 农业科学与技术(英文版)Agricultural Science ＆ Technology, 2011, 0(7) |
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| Authors: | HAN Song ZHANG Shou-cun LIN Tian-xing GONG Ming-fu .Key Laboratory of Protection & Utilization of Biological Resources in Tarim Basin of Xinjiang Production Construction Corps College of Life Science Tarim University Alar .School of Chemistry Life Sciences Leshan Normal University Leshan |
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| Affiliation: | HAN Song1,ZHANG Shou-cun1,LIN Tian-xing2,GONG Ming-fu1,2* 1.Key Laboratory of Protection & Utilization of Biological Resources in Tarim Basin of Xinjiang Production Construction Corps,College of Life Science,Tarim University,Alar 843300,2.School of Chemistry and Life Sciences,Leshan Normal University,Leshan 634004 |
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| Abstract: | [Objective] With a strain of endophytic antagonistic bacteria with good disease control efficacy in pot as trial material,the paper was to explore the antagonistic mechanism of siderophore against Fusarium oxysporum.[Method] Whether the strain produced siderophore and its fluorescent property was judged by MSA(Modified Sugar-Aspartic acid) plate.The siderophore activity of strains in liquid MSA medium was determined through the absorbance values at particular wavelength.The inhibition effects of it sideroph... |
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| Keywords: | Endophytic bacteria Siderophore Fusarium oxysporum Antagonism |
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