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| 一种纯化苏云金杆菌Cry1Ac蛋白的方法 | |
| 引用本文： | 王发祥,刘永乐,丁学知,夏立秋.一种纯化苏云金杆菌Cry1Ac蛋白的方法[J].湖南农业科学,2011(15):117-119. |
| 作者姓名： | 王发祥 刘永乐 丁学知 夏立秋 |
| 作者单位： | 1. 长沙理工大学化学与生物工程学院,长沙410114;湖南师范大学生命科学学院微生物分子生物学湖南省重点实验室,长沙410081 2. 长沙理工大学化学与生物工程学院,长沙,410114 3. 湖南师范大学生命科学学院微生物分子生物学湖南省重点实验室,长沙,410081 |
| 基金项目： | 国家“863计划”(2008AA100801); 湖南省科技支撑计划(2010NK3003); 湖南省教育厅项目(10C0400) |
| 摘 要： | Cry蛋白的纯化技术在很大程度上制约了其结构与功能的研究.为此,提出了一种以等电点沉淀方法纯化苏云金杆菌Cry1Ac的原毒素,用分子筛层析纯化其活性毒素的方法.结果表明:Cry1Ac的原毒素在pH值5.05条件下沉淀纯化效果好；以纯化的原毒素激活进行分子筛层析,收集其最大峰便可获得较高浓度的活性毒素；该方法不仅纯化效果... |
| 关 键 词： | 苏云金杆菌 Cry1Ac 纯化 等电点沉淀 分子筛层析 |
An Improved Method for Purifying Cry1Ac Protein from Bacillus thuringiensis |
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| WANG Fa-xiang,LIU Yong-le,DING Xue-zhi,XIA Li-qiu.An Improved Method for Purifying Cry1Ac Protein from Bacillus thuringiensis[J].Hunan Agricultural Sciences,2011(15):117-119. | |
| Authors: | WANG Fa-xiang LIU Yong-le DING Xue-zhi XIA Li-qiu |
| Institution: | WANG Fa-xiang1,2,LIU Yong-le1,DING Xue-zhi2,XIA Li-qiu2(1.College of Chemistry and Bioengineering,Changsha University of Science and Technology,Changsha 410114,PRC,2.College of Life Science,Hunan Normal University,Hunan Provincial Key Laboratory for Molecular Biology of Microorganism,Changsha 410081,PRC) |
| Abstract: | The research of structure and function of Cry proteins was greatly restricted by their purification method.Therefore,a method that purifying Bacillus thuringiensis Cry1Ac protoxin by using isoelectric precipitation and purifying Bacillus thuringiensis Cry1Ac activated toxin by using molecular sieve chromatography was proposed.The results showed that the Cry1Ac protoxin had good effects of precipitation and purification when pH value was 5.05;activating the purified protoxin to conduct molecular sieve chroma... |
| Keywords: | Bacillus thuringiensis Cry1Ac purification isoelectric precipitation molecular sieve chromatography |
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