Extraction Optimization of Polysaccharides from Pitaya Stems |
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Authors: | HE Cong-fen LI Peng ZHAO Hua SONG Li-ya ZHU Jun DONG Yin-mao |
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Affiliation: | HE Cong-fen1,LI Peng1,2,ZHAO Hua1,SONG Li-ya1,ZHU Jun1,DONG Yin-mao1* 1.Beijing Key Lab of Plant Resources Research and Development,Beijing Technology and Business University,Beijing 100048,2.College of Life Science,Capital Normal University,Beijing 100037 |
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Abstract: | [Objective] The aim was to describe the extraction of polysaccharides from pitaya stems.[Method] The hot water,enzyme-assisted and microwave-assisted methods were used,with the microwave-assisted extraction being deemed optimal by general evaluation.[Result] The main factors affecting the yield of polysaccharides in the microwave-assisted extraction,by order of magnitude,were as follows:time>microwave power>temperature;additionally,optimal conditions included a 10 min extraction time,an 80 ℃ extraction temperature and a microwave setting of 200 W.Using these optimal conditions,the yield of PSPS(Polysaccharides from Pitaya Stems) was 1.42%.After purification,the yield of PSPS was 0.74%.[Conclusion] The PSPS was analyzed by IR,MALDI-TOF-MS and an element analysis technique.It was shown to be a polysaccharide mixture,and the molecular weight was between 3 900 and 4 300 Da. |
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Keywords: | Stem of pitaya Polysaccharide Extraction Process optimization Structure identification |
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