| 芝麻子叶不定芽诱导关键影响因子研究 |
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| 引用本文： | 安建伟,王月华,卫双玲,郭旺珍,张海洋. 芝麻子叶不定芽诱导关键影响因子研究[J]. 河南农业科学, 2011, 40(9): 44-47,51 |
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| 作者姓名： | 安建伟 王月华 卫双玲 郭旺珍 张海洋 |
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| 作者单位： | 1. 南京农业大学作物遗传育种种质创新国家重点实验室,江苏南京,210095
2. 河南省农业科学院芝麻研究中心,河南郑州,450002 |
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| 基金项目： | 国家芝麻产业技术体系建设专项资金(CARS-15) |
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| 摘 要： | 以豫芝4号为材料,对芝麻子叶诱导不定芽形成的关键影响因子,如ABA、AgNO3、暗培养时间、碳源、琼脂质量浓度、pH值等进行了研究.结果表明,种子经振荡暗培养24h处理后,取子叶外植体置诱导及分化培养基上暗培养8d,移到光照条件下继续培养,不定芽诱导效果最好,诱导率达50％.培养基中添加5mg/L的AgNO3能防止外植...
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| 关 键 词： | 芝麻 子叶 不定芽诱导 影响因子 |
Study on Induced Factors Affecting Adventitious Bud Formation from Cotyledon in Sesame |
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| AN Jian-wei,WANG Yue-hua,WEI Shuang-ling,GUO Wang-zhen,ZHANG Hai-yang. Study on Induced Factors Affecting Adventitious Bud Formation from Cotyledon in Sesame[J]. Journal of Henan Agricultural Sciences, 2011, 40(9): 44-47,51 |
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| Authors: | AN Jian-wei WANG Yue-hua WEI Shuang-ling GUO Wang-zhen ZHANG Hai-yang |
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| Affiliation: | AN Jian-wei1,WANG Yue-hua2,WEI Shuang-ling2,GUO Wang-zhen1,ZHANG Hai-yang2(1.National Key Laboratory of Crop Genetics and Germplasm Enhancement,Nanjing AgriculturalUniversity,Nanjing 210095,China,2.Sesame Research Center,Henan Academy of Agricultural Sciences,Zhengzhou 450002,China) |
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| Abstract: | The optimal conditions for plantlet regeneration from sesame cotyledon(Yuzhi No.4) were investigated in detail.Results showed that explants of sesame cotyledon should be shaken and pre-cultured in 1/2 MS liquid medium for 24h in darkness,before being cut and cultured for adventitious bud induction.During the bud induction from cotyledons,culturing in darkness for 8d had significant advantages and subsequent differentiation should be carried out in light;Furthermore,the frequency of adventitious bud differen... |
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| Keywords: | Sesame Cotyledon Adventitious shoot induction Induced factor |
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