| miRNA-181b在牛腺垂体中的表达规律及其调控作用研究(英文) |
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| 引用本文： | 许橙,薛文志,赵茂鑫,陈信,刘颖,于汪洋,许艳丽,马腾壑,高妍,张嘉保,袁宝. miRNA-181b在牛腺垂体中的表达规律及其调控作用研究(英文)[J]. （《农业科学与技术》）编辑部, 2011, 0(7) |
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| 作者姓名： | 许橙 薛文志 赵茂鑫 陈信 刘颖 于汪洋 许艳丽 马腾壑 高妍 张嘉保 袁宝 |
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| 作者单位： | 吉林大学畜牧兽医学院;吉林大学实验动物中心; |
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| 基金项目： | Supported by National Natural Science Foundation of China(30972100); Jilin Science and Technology Development Pro-gram (201101015)~~ |
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| 摘 要： | [目的]探索miRNA-181b在成熟(18月龄)和未成熟(1月龄)牛腺垂体中差异表达量及其调控功能。[方法]通过构建成熟(18月龄)和未成熟(1月龄)的延边黄牛腺垂体组织miRNAcDNA文库,对文库中的miRNA进行Solexa高通量深度测序,并对公牛腺垂体中的miRNA进行鉴别。从测序结果中挑选出具有差异表达的miRNA-181b,经实时荧光定量PCR验证其在不同发育期延边黄牛腺垂体中的表达规律,并通过TargetScanS预测miRNA-181b的靶基因。[结果]miRNA-181b在不同发育期牛腺垂体表达量存在显著差异,1月龄公牛腺垂体中miRNA-181b表达量是18月龄的4.05倍。miRNA181b与FSHβ基因3 非编码区838 ~844碱基可能是特异结合,其结合碱基为UGAAUGUA。[结论]该研究为FSHβ的转录后调控研究提供了理论依据。
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| 关 键 词： | miRNA 实时荧光定量PCR 差异表达 |
Study on the Expression Laws and Regulatory Functions of miRNA-181b in the Anterior Pituitary of Cattle |
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| XU Cheng,XUE Wen-zhi,ZHAO Mao-xin,CHEN Xin,LIU Ying,YU Wang-yang,XU Yan-li,MA Teng-he,GAO Yan,ZHANG Jia-bao,YUAN Bao .College of Animal Science , Veterinary Medicine,Jilin University,Changchun ,.Laboratory Animal Center. Study on the Expression Laws and Regulatory Functions of miRNA-181b in the Anterior Pituitary of Cattle[J]. 农业科学与技术(英文版)Agricultural Science ＆ Technology, 2011, 0(7) |
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| Authors: | XU Cheng XUE Wen-zhi ZHAO Mao-xin CHEN Xin LIU Ying YU Wang-yang XU Yan-li MA Teng-he GAO Yan ZHANG Jia-bao YUAN Bao .College of Animal Science Veterinary Medicine Jilin University Changchun .Laboratory Animal Center |
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| Affiliation: | XU Cheng1,XUE Wen-zhi1,ZHAO Mao-xin1,CHEN Xin1,LIU Ying1,YU Wang-yang1,XU Yan-li1,MA Teng-he1,GAO Yan2,ZHANG Jia-bao2,YUAN Bao2* 1.College of Animal Science and Veterinary Medicine,Jilin University,Changchun 130062,2.Laboratory Animal Center |
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| Abstract: | [Objective] The research aimed to discuss the differential expression quantity of miRNA-181b in mature(18-month-old) and immature(one-month-old) cattle's anterior pituitary and its regulation function.[Method] cDNA library of miRNA in mature(18-month-old) and immature(one-month-old) cattle's anterior pituitary were established.After Solexa high-throughput sequencing of miRNA in the cDNA library,miRNA in anterior pituitary of bulls was identified.miRNA-181b with differential expression were selected from the... |
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| Keywords: | miRNA Real-time quantitative RT-PCR Differential expression |
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