| PDE3与亮甲酚蓝在绵羊卵母细胞体外培养中的应用研究(英文) |
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| 引用本文： | 汪立芹,王静,金贤华,陈博,黄俊成.PDE3与亮甲酚蓝在绵羊卵母细胞体外培养中的应用研究(英文)[J].Agricultural Science&Technology,2011(7). |
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| 作者姓名： | 汪立芹 王静 金贤华 陈博 黄俊成 |
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| 作者单位： | 新疆动物生物技术重点开放实验室;农业部草食家畜繁育生物技重点开放实验室; |
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| 基金项目： | Supported by Natural Science Foundation of Xinjiang AutonomousRegion (200821182 ); Science and Technology Research andDevelopment Program of Xinjiang Autonomous Region (200841122); Science and Technology Planning Project of Xinjiang AutonomousRegion (200711104); the National Transgenic Major Program~~ |
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| 摘 要： | 目的]探讨磷酸二酯酶3(PDE3)特异性抑制剂Milrinone与亮甲酚蓝染色液(BCB)相结合在绵羊卵母细胞体外培养中的应用。方法]比较了BCB对绵羊卵母细胞的筛选与传统的形态分级之间的差异,以及对胚胎后期发育的影响;研究了Milrinone对绵羊卵母细胞的最佳抑制时间,并用于BCB-卵母细胞体外培养。结果]A、B级卵母细胞中BCB+卵母细胞的比例为64.42%,极显著高于C级卵母细胞的17.00%; BCB+卵母细胞的成熟率(86.16%)、卵裂率(85.29%)、囊胚率(34.40%)分别极显著高于BCB-卵母细胞(50.94%、36.19%和6.73%);6 h是Milrinone对绵羊卵母细胞的最佳抑制时间,该试验组体外培养效率显著高于其他组;Milrinone对BCB-卵母细胞抑制培养6 h,极显著提高了体外胚胎发育率。结论]为提高绵羊卵母细胞体外培养效率提供了依据。
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| 关 键 词： | 磷酸二酯酶3 亮甲酚蓝 Milrinone 绵羊 卵母细胞 |
Application of PDE3 and Brilliant Cresyl Blue in In-vitro Culture of Sheep Oocyte |
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| WANG Li-qin,WANG Jing,JIN Xian-hua,CHEN Bo,HUANG Jun-cheng, .Key Laboratory of Animal Biotechnology of Xinjiang,Urumqi ,.Key Laboratory of Livestock Reproduction&Breed Biotechnology,Ministry of Agriculture.Application of PDE3 and Brilliant Cresyl Blue in In-vitro Culture of Sheep Oocyte[J].农业科学与技术(英文版),2011(7). |
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| Authors: | WANG Li-qin WANG Jing JIN Xian-hua CHEN Bo HUANG Jun-cheng Key Laboratory of Animal Biotechnology of Xinjiang Urumqi Key Laboratory of Livestock Reproduction&Breed Biotechnology Ministry of Agriculture |
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| Institution: | WANG Li-qin1,2,WANG Jing1,JIN Xian-hua1,CHEN Bo1,HUANG Jun-cheng1,2* 1.Key Laboratory of Animal Biotechnology of Xinjiang,Urumqi 830000,2.Key Laboratory of Livestock Reproduction&Breed Biotechnology,Ministry of Agriculture |
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| Abstract: | Objective] The aim of this study was to increase the viability of sheep oocytes in vitro by using phosphodiesterase type 3(PDE 3) inhibitor milrinone combined with brilliant cresyl blue(BCB) staining.Method] The differences between BCB tested and morphologically selected oocytes,as well as the effect of them on embryo development were compared;and then suitable inhibitive time of milrinone to sheep oocytes in vitro was studied and used in BCB-oocytes for in vitro embryo production(IVEP).Result] The BCB+ ... |
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| Keywords: | PDE3 Brilliant cresyl blue Milrinone Sheep oocytes |
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