| 转基因烟草抗性外植体培育的研究 |
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| 引用本文： | 沙琰琰,刘品,史团省,张志杰,赵青.转基因烟草抗性外植体培育的研究[J].河南农业科学,2011,40(10):52-55. |
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| 作者姓名： | 沙琰琰 刘品 史团省 张志杰 赵青 |
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| 作者单位： | 郑州大学生物工程系生物多样性与生态学研究所,河南郑州,450001 |
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| 基金项目： | 国家自然科学基金项目(30771301); 教育部留学回国人员科研启动基金项目 |
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| 摘 要： | 研究了不同培养方法、激素种类及质量浓度、抗生素种类及质量浓度对转基因烟草K326外植体分化的影响,并分别对其进行比较.结果表明:在培育转基因烟草K326抗性外植体的不同时期,依次选用最佳配方的烟草叶盘共培养培养基MS+1 mg/L 6 - BA+0.1 mg/L NAA、烟草滤菌培养基MS+1mg/L 6- BA+0....
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| 关 键 词： | 转基因烟草 外植体 培养基 转化 共培养 |
Research on Cultivation of Transgenic Tobacco's Explant |
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| SHA Yan-yan,LIU Pin,SHI Tuan-sheng,ZHANG Zhi-jie,ZHAO Qing.Research on Cultivation of Transgenic Tobacco's Explant[J].Journal of Henan Agricultural Sciences,2011,40(10):52-55. |
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| Authors: | SHA Yan-yan LIU Pin SHI Tuan-sheng ZHANG Zhi-jie ZHAO Qing |
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| Institution: | SHA Yan-yan,LIU Pin,SHI Tuan-sheng,ZHANG Zhi-jie,ZHAO Qing (The Research Institute of Biodiversity and Ecology,Department of Bioengineering,Zhengzhou University,Zhengzhou 450001,China) |
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| Abstract: | This research was to study the influence of different cultivation methods,hormones and antibiotics on explant differentiation of transgenic tobacco.There were five different culture media for the research,including MS+1 mg/L 6-BA+0.1 mg/L NAA for co-cultivation,MS+1 mg/L6-BA+0.1 mg/L NAA+500 mg/L Carb for sterilization cultivation,MS+100 mg/L Kan for screening,MS+80 mg/L Kan+300 mg/L Carb for subculture,and MS +0.1 mg/L NAA+300 mg/L Carb for root induction.With these mediums the explant of transgenic tobacc... |
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| Keywords: | Transgenic tobacco Explant Culture medium Transformation Co-cultivation |
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