| 怀地黄花药愈伤组织诱导研究 |
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| 引用本文： | 周春娥,朱凤荣,蔡兆奎,王灵灵,谷燕燕.怀地黄花药愈伤组织诱导研究[J].河南农业科学,2011,40(8):169-171. |
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| 作者姓名： | 周春娥 朱凤荣 蔡兆奎 王灵灵 谷燕燕 |
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| 作者单位： | 1. 河南师范大学生命科学学院,河南新乡,453007
2. 新乡学院,河南新乡,453001 |
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| 基金项目： | 国家高技术研究发展计划(863)项目子课题(2006AA100109)资助; 河南省教育厅自然科学研究计划项目(2009B180016)资助;河南省教育厅自然科学研究计划项目(2011B180035)资助; 河南省高校道地中药材保育及利用工程技术研究中心资助; 河南师范大学校青年科学基金资助 |
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| 摘 要： | 以怀地黄花药为材料,研究了激素、温度、品种及光照对怀地黄愈伤组织诱导的影响.结果表明:2,4-D、6 - BA、KT和品种对怀地黄愈伤组织诱导均有一定影响,各因素的组合以2,4-D 2.0mg/L+6 - BA0.5rmg/L+KT 2.0mg/L+串地龙为最佳,诱导率达到47％;4℃低温预处理3d,诱导效果最佳,诱导...
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| 关 键 词： | 怀地黄 花药 愈伤组织 诱导因素 |
Study of Influential Factors on Anther Callus Induction of Rehmannia glutinosa Libosch |
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| ZHOU Chun-e,ZHU Feng-rong,CAI Zhao-kui,WANG Ling-ling,GU Yan-yan.Study of Influential Factors on Anther Callus Induction of Rehmannia glutinosa Libosch[J].Journal of Henan Agricultural Sciences,2011,40(8):169-171. |
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| Authors: | ZHOU Chun-e ZHU Feng-rong CAI Zhao-kui WANG Ling-ling GU Yan-yan |
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| Institution: | ZHOU Chun-e1,ZHU Feng-rong2,CAI Zhao-kui1,WANG Ling-ling1,GU Yan-yan1(1.College of Life Sciences,Henan Normal University,Xinxiang 453007,China,2.Xinxiang University,Xingxiang 453001,China) |
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| Abstract: | Using the anther of Rehmannia glutinosa Libosch as materials,several factors,including different hormone treatments,temperature,cultivars,and light on anther callus induction of Rehmannia glutinosa Libosch were studied.The result showed that 2,4-D,6-BA,KT,and cultivars had important effects on anther induction.2,4-D 2.0mg/L+6-BA 0.5mg/L + KT 2.0mg/L + Chuandilong was the best combination for callus induction.The pre-treatment at 4℃ for 3 days were the most conductive to callus induction with induction rate of 43%;It was more favorable to callus induction in darkness than in light,which reduced callus browning during the later growth. |
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| Keywords: | Rehmannia glutinosa Libosch Anther culture Callus Induction factors |
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