| 小麦叶绿体DNA提取方法研究 |
| |
| 引用本文： | 侯典云,马占强,徐虹,郭蔼光.小麦叶绿体DNA提取方法研究[J].河南农业科学,2011,40(10):38-40. |
| |
| 作者姓名： | 侯典云 马占强 徐虹 郭蔼光 |
| |
| 作者单位： | 1. 河南科技大学农学院,河南洛阳,471003
2. 西北农林科技大学生命科学学院,陕西杨凌,712100 |
| |
| 基金项目： | 教育部新世纪优秀人才支持计划(02240601); 河南科技大学人才科学研究基金(09001428),河南科技大学青年科学基金(2010QN0004),河南科技大学实验技术开发基金项目(SY1011043) |
| |
| 摘 要： | 以成熟的中国春小麦叶片为材料,采用改进的高盐-低pH方法提取叶绿体DNA,经琼脂糖凝胶电泳和PCR扩增,结果表明:所提取的小麦叶绿体DNA纯度高(OD260/280=1.89,OD260/230=2.23),适于PCR反应、基因扩增和酶切鉴定等分子操作,为深入研究小麦叶绿体基因组奠定了基础。
|
| 关 键 词： | 小麦 叶绿体DNA 提取方法 高盐-低pH法 |
Study on Extraction Method of Wheat Chloroplast DNA |
| |
| HOU Dian-yun,MA Zhan-qiang,XU Hong,GUO Ai-guang.Study on Extraction Method of Wheat Chloroplast DNA[J].Journal of Henan Agricultural Sciences,2011,40(10):38-40. |
| |
| Authors: | HOU Dian-yun MA Zhan-qiang XU Hong GUO Ai-guang |
| |
| Institution: | HOU Dian-yun1,MA Zhan-qiang1,XU Hong2,GUO Ai-guang2 (1.College of Agriculture,Henan University of Science and Technology,Luoyang 471003,China,2.College of Life Sciences,Northwest A&F University,Yangling 712100,China) |
| |
| Abstract: | An improved high-salt low-pH method was used to extract chloroplast DNA from leaf tissues of wheat cultivar Chinese spring.The agrose gel electrophoresis and PCR amplification showed that wheat chloroplast DNA of high purity(OD260/280=1.89,OD260/230=2.23)wasobtained.The DNA was suitable to be used as template in PCR,gene amplification and enzymedigestion. |
| |
| Keywords: | Wheat Chloroplast DNA Extraction method High-salt low-pH method |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
