刺齿贯众的组织培养*

 对刺齿贯众孢子萌发、原叶体增殖、孢子体的诱导进行了试验、观察和分析，研究了培养基和植物激素对其生长的影响。结果显示：成熟孢子在1/2 MS蔗糖浓度为1%培养基上59d萌发90%以上，且有利于原叶体的形成。原叶体在1/2 MS+Kt 0.5 mg/L培养基上60d增殖速率可达1∶11。试管苗在1/2 MS+IBA 2.0mg/L的培养基上长势较好，根系粗壮且发达。该物种组培和快繁的成功，为它离体保存和持续利用提供了技术支撑。

Tissue Culture of Cyrtomium caryotideum

The spore germination, prothallium proliferation and sporophyte inducement of Cyrtomium caryotideum were conducted, observed and analyzed. The impact of medium and plant hormones upon its growth had been studied. The results showed that over 90% of mature spores could germinate in 1/2 MS medium with 1% sucrose for 59 d. Such medium could improve the formation of prothalli. The proliferation ratio could reach 1∶11 when prothalli were transferred in 1/2MS+Kt 0.5 mg/L medium for 60 d. The seedlings could grow well in 1/2 MS+IBA 2.0 mg/L medium, and develop brawny roots. The success of tissue culture and rapid propagation provided technical supporting for the in vitro preservation and sustainable uses of Cyrtomium caryotideum.