| 不同外源激素和培养温度对马铃薯试管薯形成的影响* |
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| 引用本文： | 蒋从莲,郭华春. 不同外源激素和培养温度对马铃薯试管薯形成的影响*[J]. 云南农业大学学报(自然科学版), 2007, 22(6): 824-828 |
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| 作者姓名： | 蒋从莲 郭华春 |
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| 作者单位： | 云南农业大学薯类作物研究所 云南昆明650201 |
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| 基金项目： | 云南省科技厅“十一五”攻关项目资助(2006NG08) |
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| 摘 要： | 以云南栽培的3个马铃薯品种（合作88、大西洋以及转心乌)的脱毒试管苗为材料，研究了不同温度以及不同的外源激素(6-BA，CCC)对试管薯形成的影响。结果表明：以MS+8% 蔗糖为基本培养基，在全黑暗条件下，加入CCC比加入BA更有利于诱导结薯。大西洋最适宜的诱导试管苗结薯的培养组合为：CCC 300 mg/L + 20 ℃；合作88为：CCC 500 mg/L + 20 ℃；转心乌则为：CCC 300 mg/L + 25 ℃。
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| 关 键 词： | 马铃薯 试管薯 矮壮素 6-苄氨基嘌呤 温度 块茎诱导 |
| 文章编号： | 1004-390X（2007）06-0824-05 |
| 收稿时间： | 2007-01-05 |
| 修稿时间： | 2007-01-05 |
Effects of Exogenous Hormones and Temperature on the Formation of Potato Micro-tuber in Vitro |
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| JIANG Cong-lian,GUO Hua-chun. Effects of Exogenous Hormones and Temperature on the Formation of Potato Micro-tuber in Vitro[J]. Journal of Yunnan Agricultural University, 2007, 22(6): 824-828 |
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| Authors: | JIANG Cong-lian GUO Hua-chun |
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| Affiliation: | Root &; Tuber Crop Research Institute, Yunnan Agricultural University, Kunming 650201,China |
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| Abstract: | Virus-free plantlets of three potato cultivars (Solanum tubersum L．) (Cooperation -88, Atlantic, Zhuanxinwu) in Yunnan Province were used as the experimental materials. The effects of different combinations of temperature and exogenous hormone(6-BA and CCC) on the formation of micro-tubers were studied．The results indicated that:the different varieties potatoes required different temperatures and concentration of exogenous hormone. CCC indicated better micro-tubers than BA in full darkness. The optional combinations for Atlantic, Cooperation -88 and Zhuanxinwu were CCC 300 mg/L + 20 ℃, CCC500 mg/L + 20 ℃ and CCC 300 mg/L + 25 ℃, respectively. |
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| Keywords: | potato micro-tuber CCC 6-BA temperature micro-tuber inducement |
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