土壤拮抗放线菌S-5210-6活性产物抑菌谱及其作用机制

为了明确拮抗性放线菌S-5210-6在植物病害生物防治中的实用价值,并为其后续的生防制剂的研发提供科学依据,在离体条件下采用生长速率法和抑菌圈法和在活体条件下采用盆栽法对其活性产物的抑菌谱进行测定。结果表明,在离体条件下S-5210-6活性产物对病原细菌具有选择性抑制作用,对供试的病原真菌均有较强的抑制作用;在温室条件下,对专性寄生病原菌黄瓜霜霉病菌,不仅能阻止其菌丝的入侵,而且对已侵入的菌丝有很好的治疗作用。采用生长速率法和孢子萌发法对S-5210-6菌株活性产物作用机制进行了研究,结果表明,S-5210-6菌株活性产物不仅能强烈抑制病原菌菌丝的生长和分生孢子的萌发,而且经S-5210-6活性产物处理的病原菌菌丝和初萌发的分生孢子,接种寄主植物后,其致病性明显减弱,病斑的扩展减慢,且致病性的减弱程度与活性产物的浓度呈正相关关系。

Inhibiting range and mechanism of the active product of S-5210-6

A study on inhibiting range and mechanism of the active product of S-5210-6 was launched for the sake of evaluating the application significance of the S-5210-6 for the biological control of plant diseases,and providing scientific basis for the follow up research on their development.The inhibiting range of the active product of S-5210-6 was studies by means of mycelium growth rate and inhibition zone in vitro and potted plant in vivo,respectively.The active product of S-5210-6 had a selective inhibition to pathogenic bacteria and a wide-range of inhibition to pathogenic fungi.It had a strong antagonistic action to 12 pathogens in vitro.To obligate parasite pathogens like Pseudoperonospron cubensis,the active product of S-5210-6 could not only hold back the infection of mycelia,but also heal the spot where infected by mycelia.The mechanism of the active product of S-5210-6 was studies.The active product of S-5210-6 had a strong inhibition of the mycelia growth and spore germination,and the pathogenicity of mycelia and spore was reduced after treated with the active product of S-5210-6.With the high concentration of the active product of S-5210-6,their pathogenicity was remarkably reduced.